LOCUS       sequence-95942-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PWP2_pCSdest
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgaagtt cgcttaccgg ttttcaaatt
      181 tgctgggtac ggtgtaccgg cgtgggaacc taaattttac ctgcaatgga aattcagtta
      241 tcagtcccgt gggcaataga gtcactgtat ttgaccttaa aaacaacaaa tctgacacgc
      301 tgcccctggc cactcggtac aacgtcaagt gcgtggggct gtccccggat ggccgcctcg
      361 ctatcatcgt cgatgaaggg ggcgatgcgc tgctggtcag cctggtctgc aggtctgtgc
      421 tgcaccactt ccacttcaag ggctctgtgc acagtgtgtc cttctcccct gatggcagga
      481 agtttgttgt cacaaagggt aacattgccc agatgtatca tgcccctggg aagaagcggg
      541 agttcaacgc cttcgttctg gacaagacct attttgggcc ctacgatgag accacctgca
      601 tcgactggac ggatgactcc aggtgctttg tggttgggag caaagacatg tccacctggg
      661 tgttcggagc cgagcgctgg gacaacctca tctactatgc actgggggga cataaggatg
      721 ccatcgtggc ctgcttcttt gaatccaaca gcctggacct gtactcactc agccaggacg
      781 gagtgctgtg catgtggcag tgtgacacg
//
