LOCUS       sequence-95945-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PDE9A_pCSdest
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag
      121 gatcacaagt ttgtacaaaa aagcaggcac catgggatcc ggctcctcca gctaccggcc
      181 caaggccatc tacctggaca tcgatggacg cattcagaag gtaatcttca gcaagtactg
      241 caactccagc gacatcatgg acctgttctg catcgccacc ggcctgcctc ggaacacgac
      301 catctccctg ctgaccaccg acgacgccat ggtctccatc gaccccacca tgcccgcgaa
      361 ttcagaacgc actccgtaca aagtgagacc tgtggccatc aagcaactct ccgagagaga
      421 agaattaatc cagagcgtgc tggcgcaggt tgcagagcag ttctcaagag cattcaaaat
      481 caatgaactg aaagctgaag ttgcaaatca cttggctgtc ctagagaaac gcgtggaatt
      541 ggaaggacta aaagtggtgg agattgagaa atgcaagagt gacattaaga agatgaggga
      601 ggagctggcg gccagaagca gcaggaccaa ctgcccctgt aagtacagtt ttttggataa
      661 ccacaagaag ttgactcctc gacgcgatgt tcccacttac cccaagtacc tgctctctcc
      721 agagaccatc gaggccctgc ggaagccgac ctttgacgtc tggctttggg agcccaatga
      781 gatgctgagc tgcctggagc acatgtacca cgacctcggg ctggtcaggg acttcagcat
      841 caaccctgtc a
//
