LOCUS       sequence-95947-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: GABPA_pCSdest
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgactaa aagagaagca gaggagctga
      181 tagaaattga gattgatgga acagagaaag cagagtgcac agaagaaagc attgtagaac
      241 aaacctacgc gccagctgaa tgtgtaagcc aggccataga catcaatgaa ccaataggca
      301 atttaaagaa actgctagaa ccaagactac agtgttcttt ggatgctcat gaaatttgtc
      361 tgcaagatat ccagctggat ccagaacgaa gtttatttga ccaaggagta aaaacagatg
      421 gaactgtaca gcttagtgta caggtaattt cttaccaagg aattgaacca aagttaaaca
      481 tccttgaaat tgttaaacct gcggacactg ttgaggttgt tattgatcca gatgcccacc
      541 atgctgaatc agaagcacat cttgttgaag aagctcaagt gataactctt gatggcacaa
      601 aacacatcac aaccatttca gatgaaactt cagaacaagt gacaagatgg gctgctgcac
      661 tggaaggcta taggaaagaa caagaacgcc ttgggatacc ctatgatccc atacagtggt
      721 ccacagacca agtcctgcat tgggtggttt gggtaatgaa ggaattcagc atgaccgata
      781 tagacctcac cacactcaac a
//
