LOCUS       sequence-95952-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DIP2A_pCSdest
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggctga ccgcgggtgc ccgctggagg
      181 cggcgccgct gcctgccgag gtgcgggaga gcctggctga gctggagctg gagctgtcgg
      241 aaggtgacat cactcaaaaa ggatatgaaa agaaaagggc aaagctgctt gcacgttata
      301 taccgcttat tcaaggaata gacccgtctc tgcaagcaga gaatagaatt cctgggccct
      361 cacaaaccac ggccgctgca cccaagcagc agaagtctcg gcccaccgcc tcgagggatg
      421 agcgcttccg gtcagatgtc cacactgaag ccgtgcaagc agctttggcc aaatacaaag
      481 agaggaagat gcctatgcct tcgaagagac gttctgtcct tgtgcattcg tctgtggaaa
      541 cctacacccc tccagacacg tcgtctgcct cagaagatga gggctcttta cggcgacccg
      601 ggcgactcac ctccactccg ctccagagcc attccagcgt cgagccctgg ctcgaccggg
      661 tcattcaggg ctcgtccacc tcatcctctg catcctccac ctcatctcac ccgggaggga
      721 gacccaccac tgctcccagt gctgcagcca cgccgggggc cgccgctacc actgcactcg
      781 caggcctcga ggcccacacc cacatagatc tgcattctg
//
