LOCUS       sequence-95956-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: FAM3B_pCSdest
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgcgccc attggctggt ggcctgctca
      181 aggtggtgtt cgtggtcttc gcctccttgt gtgcctggta ttcggggtac ctgctcgcag
      241 agctcattcc agatgcaccc ctgtccagtg ctgcctatag catccgcagc atcggggaga
      301 ggcctgtcct caaagctcca gtccccaaaa ggcaaaaatg tgaccactgg actccctgcc
      361 catctgacac ctatgcctac aggttactca gcggaggtgg cagaagcaag tacgccaaaa
      421 tctgctttga ggataaccta cttatgggag aacagctggg aaatgttgcc agaggaataa
      481 acattgccat tgtcaactat gtaactggga atgtgacagc aacacgatgt tttgatatgt
      541 atgaaggcga taactctgga ccgatgacaa agtttattca gagtgctgct ccaaaatccc
      601 tgctcttcat ggtgacctat gacgacggaa gcacaagact gaataacgat gccaagaatg
      661 ccatagaagc acttggaagt aaagaaatca ggaacatgaa attcaggtct agctgggtat
      721 ttattgcagc aaaaggcttg gaactccctt ccgaaattca gagagaaaag atcaaccact
      781 ctgatgctaa gaacaacaga tattctggct ggcctgcaga gatccagata gaaggctgca
      841 ta
//
