LOCUS       sequence-95957-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Yount JS, Karssemeijer RA, Hang HC
  TITLE     S-palmitoylation and ubiquitination differentially regulate 
            interferon-induced transmembrane protein 3 (IFITM3)-mediated 
            resistance to influenza virus.
  JOURNAL   J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 
            10.1074/jbc.M112.362095. Epub 2012 Apr 17.
  PUBMED    22511783
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362095"; journalName: "J Biol Chem"; date: 
            "2012-06-1- 1"; volume: "287"; issue: "23"; pages: "19631-41"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-K83,88,104A
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..68
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    546..680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(570..589)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     624..643
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(791..807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(791..807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(804..826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    815..831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(839..845,846..863,864..869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac gatgttccag
       61 attacgctct tatggccatg gaggcccgaa ttcccatgaa ccacacttct caagccttca
      121 tcaccgctgc cagtggagga cagcccccaa actacgaaag aatcaaggaa gaatatgagg
      181 tggctgagat gggggcaccg cacggatcgg cttctgtcag aactactgtg atcaacatgc
      241 ccagagaggt gtcggtgcct gaccatgtgg tctggtccct gttcaataca ctcttcatga
      301 acttctgctg cctgggcttc atagcctatg cctactccgt ggcgtctagg gatcgggcga
      361 tggtgggtga tgtgactgga gcccaggcct acgcctccac tgctgcgtgc ctgaacatca
      421 gcaccttggt cctcagcatc ctgatggttg ttatcaccat tgttagtgtc atcatcattg
      481 ttcttaacgc tcaaaacctt cacacttaag tcgaccgaga tctctcgagg taccgcggcc
      541 gcggggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      601 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      661 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      721 gggaggtgtg ggaggttttt tcggatcctc tagagtcgat ctgcaggcat gctagcttgg
      781 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      841 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      901 c
//
