LOCUS       sequence-95959-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ArchT-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4ArchT-mCherry
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..806)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(377..396)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     646..664
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agtctcaggt tgagaagtgt tgccagttgt tcttctgatc taatgttttt ttctcgactg
       61 aaaagcttag atgacccagc actttataac ccgggtcatt acttgtacag ctcgtccatg
      121 ccgccggtag agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
      181 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
      241 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
      301 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
      361 gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
      421 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
      481 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
      541 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
      601 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
      661 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
      721 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
      781 atgttatcct cctcgccctt gctcactgct actaccggtc gg
//
