LOCUS       sequence-95966-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-P2A-B19G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synP-FLEX-EGFP-B19G
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(411..444)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(495..528)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg
       61 catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca
      121 gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc
      181 agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac
      241 cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata
      301 ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct
      361 gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcgaattc ataacttcgt
      421 atagcataca ttatacgaag ttattctttg caccattcta aagaataaca gtgataattt
      481 ctgggttaag gcaaataact tcgtatagga tactttatac gaagttatgc tagcttacag
      541 tctggtctca cccccactct tgtgtgattc ccatgaagat atgatcttcc cgctttgggg
      601 agtgactgac acctccctcc ctgtccctct gagattgtgt tgcgtaggtt ctgatcgatt
      661 gactcttcta caacatgtca tcaggaaaat tatcaacatc aaggcagtca gggcccctgc
      721 actcagtaat acatact
//
