LOCUS       sequence-95978-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of dsRed and constitutive expression of GFP in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW
  TITLE     Dynamics of intracellular bacterial replication at the single cell 
            level.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51. doi: 
            10.1073/pnas.1000041107. Epub 2010 Feb 2.
  PUBMED    20133586
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1000041107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-02-23- 23"; volume: "107"; issue: "8"; pages: "3746-51"
COMMENT     Sequence Label: pDiGc
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(299..319)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 attgacggct tgacggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag
       61 gcacattatg catcgtcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
      121 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
      181 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttgcatgcc
      241 tgcaggtctg gacatttatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt
      301 tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga
      361 ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
      421 ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa
      481 gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata
      541 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa
      601 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt
      661 cccgtcatct ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt
      721 gaaaaagtca tgctgtttca tatgatctgg gtatctcgca aagcattgaa caccataacc
      781 gaaagtagtg acaagtgttg g
//
