LOCUS       sequence-95981-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PSMG1_pCSdest
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggcggc cacgttcttc ggagaggtgg
      181 tgaaggcgcc gtgccgagct gggactgagg acgaagagga ggaggaggag gggcggaggg
      241 agacgcccga ggacagggag gtgcgtctgc agctggcgcg gaagagggaa gtgcggctcc
      301 ttcgaagaca aacaaaaaca tctttggaag tttctttgct agaaaaatat ccgtgctcca
      361 agtttataat tgctatagga aataatgcag tagcatttct gtcatcattt gttatgaatt
      421 caggagtctg ggaggaagtt ggttgtgcta aactctggaa tgaatggtgt agaacaacag
      481 acactacaca tctgtcctcc acagaggctt tttgtgtgtt ttatcatcta aaatccaatc
      541 cctcggtttt tctctgtcag tgcagttgct atgttgcaga agatcaacag tatcagtggc
      601 tggaaaaggt ttttggctct tgtccaagga agaacatgca gataactatt ctcacatgtc
      661 gacatgttac cgattataaa acctcagaat ccaccggcag ccttccttct cctttcctga
      721 gagccctaaa aacacagaat ttcaaagact cggcgtgttg tccattgcta gaacaaccga
      781 atatagtaca cgaccttcct gcagcagttc taagctactg tc
//
