LOCUS       sequence-95990-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian 
            cells (mutates possible glycosylation site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Yount JS, Karssemeijer RA, Hang HC
  TITLE     S-palmitoylation and ubiquitination differentially regulate 
            interferon-induced transmembrane protein 3 (IFITM3)-mediated 
            resistance to influenza virus.
  JOURNAL   J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 
            10.1074/jbc.M112.362095. Epub 2012 Apr 17.
  PUBMED    22511783
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362095"; journalName: "J Biol Chem"; date: 
            "2012-06-1- 1"; volume: "287"; issue: "23"; pages: "19631-41"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-N2A
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    520..654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(544..563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     598..617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgcgggccca ccatgtaccc atacgatgtt ccagattacg ctcttatggc catggaggcc
       61 cgaattccca tggcacacac ttctcaagcc ttcatcaccg ctgccagtgg aggacagccc
      121 ccaaactacg aaagaatcaa ggaagaatat gaggtggctg agatgggggc accgcacgga
      181 tcggcttctg tcagaactac tgtgatcaac atgcccagag aggtgtcggt gcctgaccat
      241 gtggtctggt ccctgttcaa tacactcttc atgaacttct gctgcctggg cttcatagcc
      301 tatgcctact ccgtgaagtc tagggatcgg aagatggtgg gtgatgtgac tggagcccag
      361 gcctacgcct ccactgctaa gtgcctgaac atcagcacct tggtcctcag catcctgatg
      421 gttgttatca ccattgttag tgtcatcatc attgttctta acgctcaaaa ccttcacact
      481 taagtcgacc gagatctctc gaggtaccgc ggccgcgggg atccagacat gataagatac
      541 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      601 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      661 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttt
//
