LOCUS       sequence-95991-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian 
            cells (mutates possible glycosylation site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Yount JS, Karssemeijer RA, Hang HC
  TITLE     S-palmitoylation and ubiquitination differentially regulate 
            interferon-induced transmembrane protein 3 (IFITM3)-mediated 
            resistance to influenza virus.
  JOURNAL   J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 
            10.1074/jbc.M112.362095. Epub 2012 Apr 17.
  PUBMED    22511783
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362095"; journalName: "J Biol Chem"; date: 
            "2012-06-1- 1"; volume: "287"; issue: "23"; pages: "19631-41"
COMMENT     Sequence Label: pCMV-HA-mIFITM3-N2A
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(488..514)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          578..674
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(791..815)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(812..863)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(841..861)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtctggatc cccgcggccg cggtacctcg agagatctcg gtcgacttaa gtgtgaaggt
       61 tttgagcgtt aagaacaatg atgatgacac taacaatggt gataacaacc atcaggatgc
      121 tgaggaccaa ggtgctgatg ttcaggcact tagcagtgga ggcgtaggcc tgggctccag
      181 tcacatcacc caccatcttc cgatccctag acttcacgga gtaggcatag gctatgaagc
      241 ccaggcagca gaagttcatg aagagtgtat tgaacaggga ccagaccaca tggtcaggca
      301 ccgacacctc tctgggcatg ttgatcacag tagttctgac agaagccgat ccgtgcggtg
      361 cccccatctc agccacctca tattcttcct tgattctttc gtagtttggg ggctgtcctc
      421 cactggcagc ggtgatgaag gcttgagaag tgtgtgccat gggaattcgg gcctccatgg
      481 ccataagagc gtaatctgga acatcgtatg ggtacatggt gggcccgcgg gtacaattcc
      541 gcagctttta gagcagaagt aacacttccg tacaggccta gaagtaaagg caacatccac
      601 tgaggagcag ttctttgatt tgcaccacca ccggatccgg gacctgaaat aaaagacaaa
      661 aagactaaac ttaccagtta actttctggt ttttcagttc ctctagtacc ggatcctcta
      721 gagtccggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc
      781 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
      841 cacgcctacc gcccatttgc gtcaatgggg
//
