LOCUS       sequence-95997-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM3-C71,72,105A with an N-terminal 
            myristoylation sequence from HIV Gag followed by an HA tag, as well 
            as a C-terminal CaaX prenylation motif, in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Yount JS, Karssemeijer RA, Hang HC
  TITLE     S-palmitoylation and ubiquitination differentially regulate 
            interferon-induced transmembrane protein 3 (IFITM3)-mediated 
            resistance to influenza virus.
  JOURNAL   J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 
            10.1074/jbc.M112.362095. Epub 2012 Apr 17.
  PUBMED    22511783
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362095"; journalName: "J Biol Chem"; date: 
            "2012-06-1- 1"; volume: "287"; issue: "23"; pages: "19631-41"
COMMENT     Sequence Label: pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..60
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(574..593)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     628..647
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cccaccatgg gggcacgggc aagcgtcttg tcttacccat acgatgttcc agattacgct
       61 cttatggcca tggaggcccg aattcccatg aaccacactt ctcaagcctt catcaccgct
      121 gccagtggag gacagccccc aaactacgaa agaatcaagg aagaatatga ggtggctgag
      181 atgggggcac cgcacggatc ggcttctgtc agaactactg tgatcaacat gcccagagag
      241 gtgtcggtgc ctgaccatgt ggtctggtcc ctgttcaata cactcttcat gaacttcgca
      301 gcactgggct tcatagccta tgcctactcc gtgaagtcta gggatcggaa gatggtgggt
      361 gatgtgactg gagcccaggc ctacgcctcc actgctaagg cactgaacat cagcaccttg
      421 gtcctcagca tcctgatggt tgttatcacc attgttagtg tcatcatcat tgttcttaac
      481 gctcaaaacc ttcacacttg cttggtcttg taagtcgacc gagatctctc gaggtaccgc
      541 ggccgcgggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      661 attat
//
