LOCUS       sequence-96030-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Hach JC, McMichael T, Chesarino NM, Yount JS
  TITLE     Palmitoylation on conserved and nonconserved cysteines of murine 
            IFITM1 regulates its stability and anti-influenza A virus activity.
  JOURNAL   J Virol. 2013 Sep;87(17):9923-7. doi: 10.1128/JVI.00621-13. Epub 
            2013 Jun 26.
  PUBMED    23804635
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00621-13"; journalName: "J Virol"; date: "2013-09"; 
            volume: "87"; issue: "17"; pages: "9923-7"
COMMENT     Sequence Label: pCMV-HA-mIFITM1-C50A
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(394..420)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          484..580
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(697..721)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(718..817)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(747..767)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtctggatcc ccgcggccgc ggtacctcga gagatctcgg tcgactcatc taatggcaca
       61 gacaacgatg acgacgatgg ccgtgaggat ggtgaagaac agggagctga tgttcaggca
      121 cttggcggtg gaggcgaagg cctgggcccc agtcgtatca cccaccatct tcctgtccct
      181 agacttcacg gagtaggcat aggctacgaa gcccagggcg cagaagttca tgaagagtgt
      241 attgaacagg gaccagacca catggtcagg cgtggagatc tcaggcatgt tgattgtggt
      301 ggctgtcgca gaagttgaga tgtggggtga ccccagtaca accacctctt gctgctcctt
      361 aggcatggga attcgggcct ccatggccat aagagcgtaa tctggaacat cgtatgggta
      421 catggtgggc ccgcgggtac aattccgcag cttttagagc agaagtaaca cttccgtaca
      481 ggcctagaag taaaggcaac atccactgag gagcagttct ttgatttgca ccaccaccgg
      541 atccgggacc tgaaataaaa gacaaaaaga ctaaacttac cagttaactt tctggttttt
      601 cagttcctct agtaccggat cctctagagt ccggaggctg gatcggtccc ggtgtcttct
      661 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
      721 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      781 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      841 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgct
//
