LOCUS       sequence-96044-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Hach JC, McMichael T, Chesarino NM, Yount JS
  TITLE     Palmitoylation on conserved and nonconserved cysteines of murine 
            IFITM1 regulates its stability and anti-influenza A virus activity.
  JOURNAL   J Virol. 2013 Sep;87(17):9923-7. doi: 10.1128/JVI.00621-13. Epub 
            2013 Jun 26.
  PUBMED    23804635
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00621-13"; journalName: "J Virol"; date: "2013-09"; 
            volume: "87"; issue: "17"; pages: "9923-7"
COMMENT     Sequence Label: pCMV-HA-mIFITM1-C83A
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    451..585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(475..494)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     529..548
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(696..712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(696..712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(709..731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    720..736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(744..750,751..768,769..774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    789..810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctctaaaagc tgcggaattg tacccgcggg cccaccatgt acccatacga tgttccagat
       61 tacgctctta tggccatgga ggcccgaatt cccatgccta aggagcagca agaggtggtt
      121 gtactggggt caccccacat ctcaacttct gcgacagcca ccacaatcaa catgcctgag
      181 atctccacgc ctgaccatgt ggtctggtcc ctgttcaata cactcttcat gaacttctgc
      241 tgcctgggct tcgtagccta tgcctactcc gtgaagtcta gggacaggaa gatggtgggt
      301 gatacgactg gggcccaggc cttcgcctcc accgccaagg ccctgaacat cagctccctg
      361 ttcttcacca tcctcacggc catcgtcgtc atcgttgtct gtgccattag atgagtcgac
      421 cgagatctct cgaggtaccg cggccgcggg gatccagaca tgataagata cattgatgag
      481 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      541 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      601 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag
      661 tcgatctgca ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttcca
//
