LOCUS       sequence-96056-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in 
            mammalian cells (cysteine-less mutant that is not palmitoylated)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Hach JC, McMichael T, Chesarino NM, Yount JS
  TITLE     Palmitoylation on conserved and nonconserved cysteines of murine 
            IFITM1 regulates its stability and anti-influenza A virus activity.
  JOURNAL   J Virol. 2013 Sep;87(17):9923-7. doi: 10.1128/JVI.00621-13. Epub 
            2013 Jun 26.
  PUBMED    23804635
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00621-13"; journalName: "J Virol"; date: "2013-09"; 
            volume: "87"; issue: "17"; pages: "9923-7"
COMMENT     Sequence Label: pCMV-HA-mIFITM1-C49,50,83,103A
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    427..561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(451..470)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     505..524
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(672..688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(672..688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(685..707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    696..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(720..726,727..744,745..750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    765..786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgcgggccca ccatgtaccc atacgatgtt ccagattacg ctcttatggc catggaggcc
       61 cgaattccca tgcctaagga gcagcaagag gtggttgtac tggggtcacc ccacatctca
      121 acttctgcga cagccaccac aatcaacatg cctgagatct ccacgcctga ccatgtggtc
      181 tggtccctgt tcaatacact cttcatgaac ttcgccgccc tgggcttcgt agcctatgcc
      241 tactccgtga agtctaggga caggaagatg gtgggtgata cgactggggc ccaggccttc
      301 gcctccaccg ccaaggccct gaacatcagc tccctgttct tcaccatcct cacggccatc
      361 gtcgtcatcg ttgtcgccgc cattagatga gtcgaccgag atctctcgag gtaccgcggc
      421 cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      481 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      541 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      601 ggggaggtgt gggaggtttt ttcggatcct ctagagtcga tctgcaggca tgctagcttg
      661 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      721 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      781 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      841 cattaatgaa tcggccaacg cgcgggg
//
