LOCUS       sequence-96093-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible conditional shRNA expression vector; TetO
            flanked H1 RNA gene promoter; Gentamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-THT III
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..181
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    join(234..252,253..275,276..294,295..317,318..336,337..359,
                     360..378,379..401,402..420,421..443,444..462,463..485,
                     486..504)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     680..699
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    744..762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
       61 ctcgcgttat cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg
      121 ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg
      181 tcggcgcgcc cacccttgga tcgatccggc cttgccggcc tcgagtttac cactccctat
      241 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      301 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      361 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      421 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      481 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggctgcagga
      541 attcgaacgc tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg tcactaggcg
      601 ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca ggggagtggc
      661 gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatgtc
      721 tttggatttg ggaatcttat aagtccctat cagtgataga gatctgcaca ctggtatttc
      781 ggtttttggg gccgcggggc
//
