LOCUS       sequence-96098-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible conditional shRNA expression vector; TetO
            flanked H1 RNA gene promoter; Gentamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-THT III
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     179..201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     193..210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     194..210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    226..325
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(397..417)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tggatttatc gcggcctcga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
       61 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
      121 taacatcaga gattttgaga cacgggccag agctgcagaa cgctagcatg gatgttttcc
      181 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
      241 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
      301 ccaactttgt acaaaaaagc aggctccgcg gccctgatca agcttcccgg gaattctttg
      361 tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg
      421 tggtctctct tttcgttggg atctttcgaa agggcagatt gataggacag gtaatggttg
      481 tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg atcagcgagt
      541 tgcacgccgc cgtcttcgat gttgtggcgg gtcttgaagt tggctttgat gccgttcttt
      601 tgcttgtcgg ccatgatgta tacgttgtgg gagttgtagt tgtattccaa cttgtggccg
      661 aggatgtttc cgtcctcctt gaaatcgatt cccttaagct cgatcctgtt gacgagggtg
      721 tctccctcaa acttgacttc agcacgtgtc ttgtagttcc cgtcgtcctt gaagaagatg
      781 gtcctctcct gcacgtatcc ctcaggcatg gcgctcttg
//
