LOCUS       sequence-96101-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible conditional shRNA expression vector; TetO
            flanked H1 RNA gene promoter; Gentamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-THT III
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          77..280
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(100..120)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    678..696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(741..760)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 atggtgtctg tttgaggttg ctagtgaaca cagttgtgtc agaagcaaat gtaagcaagc
       61 taattcctca cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga
      121 gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc
      181 aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc
      241 cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg aggtgcctgg
      301 acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt
      361 tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc
      421 tccctgcagc agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg
      481 ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagagcag
      541 gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc
      601 tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg
      661 aaataccagt gtgcagatct ctatcactga tagggactta taagattccc aaatccaaag
      721 acatttcacg tttatggtga tttcccagaa cacatagcga catgcaaata ttgcagggcg
      781 ccactcccct gtccctcaca gccatcttcc tgccagggcg cacgcgcgct
//
