LOCUS       sequence-96104-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible conditional shRNA expression vector; TetO
            flanked H1 RNA gene promoter; Kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-THT
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..102
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     94..111
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     95..111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    127..226
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(298..318)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
       61 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
      121 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      181 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccgc ggccctgatc
      241 aagcttcccg ggaattcttt gtatagttca tccatgccat gtgtaatccc agcagctgtt
      301 acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggcagat
      361 tgataggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
      421 tgttgataat gatcagcgag ttgcacgccg ccgtcttcga tgttgtggcg ggtcttgaag
      481 ttggctttga tgccgttctt ttgcttgtcg gccatgatgt atacgttgtg ggagttgtag
      541 ttgtattcca acttgtggcc gaggatgttt ccgtcctcct tgaaatcgat tcccttaagc
      601 tcgatcctgt tgacgagggt gtctccctca aacttgactt cagcacgtgt cttgtagttc
      661 ccgtcgtcct tgaagaagat ggtcctctcc tgcacgtatc cctcaggcat ggcgctcttg
      721 aagaagtcgt gccgcttcat atgatctggg tatcttgcga agcattgtac accataagtg
      781 agagtagtga caagtgttgg cca
//
