LOCUS       sequence-96118-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  TITLE     NFkappaB EMSA Studies
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p50 cFlag pcDNA3
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttatctagac tcgagccacc atggcagacg
      121 atgatcccta cggaactggg caaatgtttc atttgaacac tgctttgact cactcaatat
      181 ttaatgcaga attatattca ccagaaatac cactgtcaac agatggccca taccttcaaa
      241 tattagagca accaaaacag aggggatttc gattccgcta tgtgtgtgaa ggcccatcac
      301 acggagggct tccgggagcc tctagtgaga agaacaagaa atcctaccca caggtcaaaa
      361 tttgcaacta tgtggggcct gcaaaggtta tcgttcagtt ggtcacaaat ggaaaaaaca
      421 tccacctgca cgcccacagc ctggtgggca agcactgtga ggacggggta tgcaccgtaa
      481 cagcaggacc caaggacatg gtggttggct ttgcaaacct gggaatactt catgtgacta
      541 agaaaaaggt atttgaaaca ctggaagcac ggatgacaga ggcgtgtatt aggggctata
      601 atcctggact tctggtgcat tctgaccttg cctatctaca agcagaaggc ggaggagacc
      661 ggcaactcac agacagagag aaggagatca tccgccaggc agccgtgcag cagaccaagg
      721 agatggacct gagcgtggtg cgcctcatgt tcacagcctt cctccctgac agcactggca
      781 gcttcactcg gagactggag cctgtggtgt cagacgccat ctatgatagc aaagccccga
      841 atgcatccaa cctgaaaatc gtgagaatgg acagaacagc aggatgtgtg acgggagggg
      901 aggagattta ccttctctgt gacaaggttc agaaagatga catccagatt cggttttatg
      961 aagaggaaga aaatggcgga gtttgggaag gatttgggga cttttccccc acggatgttc
     1021 atagacagtt tgccattgtc ttcaaaacgc caaagtataa ggatgtcaac attacaaagc
     1081 c
//
