LOCUS       sequence-96120-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible conditional shRNA expression vector; TetO
            flanked H1 RNA gene promoter; Kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-THT
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          96..299
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(119..139)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    697..715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(760..779)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 aagttcttct cctttaccca tggtgtctgt ttgaggttgc tagtgaacac agttgtgtca
       61 gaagcaaatg taagcaagct aattcctcac gacacctgaa atggaagaaa aaaactttga
      121 accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag
      181 gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga
      241 aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagctcgag
      301 aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct
      361 gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc
      421 cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct ttccgctcac
      481 gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg
      541 aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat tctcggtggc
      601 cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg
      661 cccgcggccc caaaaaccga aataccagtg tgcagatctc tatcactgat agggacttat
      721 aagattccca aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac
      781 atgcaaatat tgcagggcgc cactcccctg tccctcacag ccatcttcct gcca
//
