LOCUS       sequence-96124-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  TITLE     NFkappaB EMSA Studies
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p50 cFlag pcDNA3
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2..19
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(33..56)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catttaggtg acactataga atagaattct tacttgtcat cgtcgtcctt gtagtcggat
       61 ccggtgaccc ctgcgttgga tttcgtgact ccgggatgga atgtaatccc accgtaggga
      121 ggaaatccgt agttcgagta gccataccct gggccagggc ttccggtact ccctccgcca
      181 ccgccactac cgaacatgcc tccaccaccg gctcccgctc cactgccgcc gccgaagctg
      241 tccgagaagt tcggcataag cttctggcgt ttcctttgca cttcctcttt gtctttgatt
      301 tcagggtagt agagaaaggg tttcggttca ctagtttcca ggtctgattt cctccgaagc
      361 tgaacaaaca cggaagctgg ctttgtaatg ttgacatcct tatactttgg cgttttgaag
      421 acaatggcaa actgtctatg aacatccgtg ggggaaaagt ccccaaatcc ttcccaaact
      481 ccgccatttt cttcctcttc ataaaaccga atctggatgt catctttctg aaccttgtca
      541 cagagaaggt aaatctcctc ccctcccgtc acacatcctg ctgttctgtc cattctcacg
      601 attttcaggt tggatgcatt cggggctttg ctatcataga tggcgtctga caccacaggc
      661 tccagtctcc gagtgaagct gccagtgctg tcagggagga aggctgtgaa catgaggcgc
      721 accacgctca ggtccatctc cttggtctgc tgcacggctg cctggcggat gatctccttc
      781 tctctgtctg tgagttgccg gtctcctccg ccttctgctt gtagataggc aaggtcagaa
      841 tgcaccagaa gtccaggatt atagccccta atacacgcct ctgtcatccg tgcttccagt
      901 gtttcaaata cctttttctt agtcacatga agtattccca ggtttgcaaa gccaaccacc
      961 atgtccttgg gtcctgctgt tacggtgcat accccgtcct cacagtgctt gcccaccagg
     1021 ctgtgggcgt gcaggtggat gttttt
//
