LOCUS       sequence-96136-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible shRNA expression vector; H1 RNA gene 
            promoter; Kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-H1
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    160..259
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        289..503
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     424..443
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      121 agagattttg agacacgggc cagagctgca gctggatggc aaataatgat tttattttga
      181 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact
      241 ttgtacaaga aagctgggtc tagatatctc gagtgcggcc gcgaattcga acgctgacgt
      301 catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc
      361 gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc
      421 atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc
      481 ttataagttc tgtatgagac cacagatctg cacactggta tttcggtttt tggggccgcg
      541 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
      601 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
      661 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
      721 gcgtgagcgg aaagatggcc gcttcccggc cctgct
//
