LOCUS       sequence-96138-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral bicistronic expression vector for TetR-KRAB and 
            Blasticidin S resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pLIB-TetR-KRAB-IRES-BLAS
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             54..674
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(127..146)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             675..695
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattaata cgactcacta taggaattca accatggcta
       61 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
      121 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
      181 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
      241 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
      301 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
      361 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
      421 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
      481 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
      541 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
      601 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
      661 gtgaaagtgg gtcgccaaaa aagaagagaa aggtcgacgg cggtggtgct ttgtctcctc
      721 agcactctgc tgtcactcaa ggaagtatca tcaagaacaa ggagggcatg gatgctaagt
      781 cactaactgc ctggtcccgg acactggtga ccttcaagga tgta
//
