LOCUS       sequence-96144-e         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral bicistronic expression vector for TetR-KRAB and 
            Blasticidin S resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pLIB-TetR-KRAB-IRES-BLAS
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             111..305
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
ORIGIN
        1 gaaaggtcga cggcggtggt gctttgtctc ctcagcactc tgctgtcact caaggaagta
       61 tcatcaagaa caaggagggc atggatgcta agtcactaac tgcctggtcc cggacactgg
      121 tgaccttcaa ggatgtattt gtggacttca ccagggagga gtggaagctg ctggacactg
      181 ctcagcagat cgtgtacaga aatgtgatgc tggagaacta taagaacctg gtttccttgg
      241 gttatcagct tactaagcca gatgtgatcc tccggttgga gaagggagaa gagccctggc
      301 tggtggagag agaaattcac caagagaccc atcctgattc agagactgca tttgaaatca
      361 aatcatcagt ttaaggatcc aagcttccta gggtcgacgc ggccgccaat tccccctctc
      421 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
      481 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
      541 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatg
//
