LOCUS       sequence-96147-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) 
            cDNA allele A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   McBride CS, Baier F, Omondi AB, Spitzer SA, Lutomiah J, Sang R, 
            Ignell R, Vosshall LB
  TITLE     Evolution of mosquito preference for humans linked to an odorant 
            receptor.
  JOURNAL   Nature. 2014 Nov 13;515(7526):222-7. doi: 10.1038/nature13964.
  PUBMED    25391959
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-11-13- 13"; volume: "515"; issue: "7526"; 
            pages: "222-7"
COMMENT     Sequence Label: pUASTattb AaegOr4-A
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..179
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    212..306
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        325..563
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     510..531
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
       61 tttatatata tcaaaaataa caactataat aataagaata catttaattt agaaaatgct
      121 tggatttcac tggaactagg ctagcataac ttcgtataat gtatgctata cgaagttatg
      181 ctagcggatc caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
      241 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
      301 tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt ctacggagcg
      361 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
      421 aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta
      481 aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta
      541 ttgaatacaa gaagagaact ctgaataggg aattgggaat tcatgactca atcccttgag
      601 tttgaccaaa cctttggctt catcgccaag gtcttgcaaa tgatcggtta tccaagctgt
      661 ttagcaccgt acccaacgac cttcgccagt cgactcaagt ccagtg
//
