LOCUS       sequence-96165-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing four copies of the Smad binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: SBE4-Luc
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(77..273)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      91..226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    382..473
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(414..433)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    487..535
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta
       61 ccggaatgcc aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
      121 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
      181 gatggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
      241 gcgggactat ggttgctgac taattgagat gcagatcgca gatctcgagc ccgggctagc
      301 acgcgtaaga gctcggtacg tctagactgc cgtctagact tagtacgtct agactgccgt
      361 ctagacttag tacctatcga tagagaaatg ttctggcacc tgcacttgca ctggggacag
      421 cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta
      481 tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattgcggc
      541 cgctccaagt acctcccgta ccttaatatt acttacttat catggtagct tgggctggcg
      601 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      661 atggcaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      721 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatca
//
