LOCUS       sequence-96167-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  self-inactivating lentiviral plasmid for co-expression of your gene 
            of interest and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z
  TITLE     Lentiviral transduction of mammary stem cells for analysis of gene 
            function during development and cancer.
  JOURNAL   Cell Stem Cell. 2008 Jan 10. 2(1):90-102.
  PUBMED    18371425
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Jan 10. 2(1):90-102."
COMMENT     Sequence Label: pHIV-EGFP
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(32..40,41..43,44..618)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(205..224)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     434..451
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(696..716)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcacca tggttgtggc catattatca tcgtgttttt
       61 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa
      121 acacatgtaa agcatgtgta ccgaggcccc agatcagatc ccatacaatg gggtaccttc
      181 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca
      241 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca
      301 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg
      361 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca
      421 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag
      481 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat
      541 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg
      601 gggggggagg gagaggggcg gatcctagcc cgggtactct agaatagtta actcagcggc
      661 cgctcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat
      721 gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc
      781 tggcctaact tca
//
