LOCUS       sequence-96172-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
  TITLE     The Src homology 3 domain is required for junctional adhesion 
            molecule binding to the third PDZ domain of the scaffolding protein 
            ZO-1.
  JOURNAL   J Biol Chem. 2011 Dec 16;286(50):43352-60. doi: 
            10.1074/jbc.M111.304089. Epub 2011 Oct 26.
  PUBMED    22030391
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.304089"; journalName: "J Biol Chem"; date: 
            "2011-12-16- 16"; volume: "286"; issue: "50"; pages: "43352-60"
COMMENT     Sequence Label: pET14b_tev_PSG
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             147..164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             180..200
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     RBS             656..664
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggccacgatg cgtccgggcg ctagaggatc gtagatcatc gatcccgcga aacttaatac
       61 gactcactat agggagacca caacggcttt ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc ggcggtaacg
      181 agaatcttta ttttcagggc catatgattc ttcggcccag catgaaattg gtaaaattca
      241 gaaaaggaga tagtgtgggt ttgcggctgg ctggtggaaa tgatgttgga atatttgtag
      301 ctggcgttct agaagatagc cctgcagcca aggaaggctt agaggaaggt gatcaaattc
      361 tcagggtaaa caacgtagat tttacaaata tcataagaga agaagccgtc cttttcctgc
      421 ttgacctccc taaaggagaa gaagtgacca tattggctca gaagaagaag gatgtttatc
      481 gtcgcattgt agaatcagat gtaggagatt ctttctatat tagaacccat tttgaatatg
      541 aaaaggaatc tccctatgga cttagtttta acaaaggaga ggtgttccgt gttgtggata
      601 ccttgtacaa tggaaaactg ggctcttggc ttgctattcg aattggtaaa aatcataagg
      661 aggtagaacg aggcatcatc cctaataaga acagagctga gcagctagcc agtgtacag
//
