LOCUS       sequence-96174-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
  TITLE     The Src homology 3 domain is required for junctional adhesion 
            molecule binding to the third PDZ domain of the scaffolding protein 
            ZO-1.
  JOURNAL   J Biol Chem. 2011 Dec 16;286(50):43352-60. doi: 
            10.1074/jbc.M111.304089. Epub 2011 Oct 26.
  PUBMED    22030391
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.304089"; journalName: "J Biol Chem"; date: 
            "2011-12-16- 16"; volume: "286"; issue: "50"; pages: "43352-60"
COMMENT     Sequence Label: pET14b_tev_PSG
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             723..731
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gttagcagcc ggatcctcat ccctcggaaa cccataccag ctggttttgc tgttgttgaa
       61 ttgcttcttt cagcgcacca taccaaccat cattcattga atttaagtta attgtagttg
      121 taaaaagatg gtgattattt ttacgaagtt tatgagatcg ctcgtataac ttcctggcac
      181 ttttccgaga ttctggacat aacctcattc tcattgtttt tactccttgc ttagaatcag
      241 ggttaagaaa tacaacaatt ggataccact gggcatagtt aagacgatca actgcatttg
      301 gtgttacatc taataatgca tgcttgtctt gatctatgat ttgctttatt gtatgcaggc
      361 gaataatgcc agagctacgt tggtcagttc cagcgtctcg tggttcactc tttgcaattt
      421 gataaatatc tggttcttct cttgccagct tttctctggc aacatcagct attggtccaa
      481 aaatggttac aggcctcaga aatccagctt ctcgaagaac cactctttca taagctggaa
      541 actttgtttg aacaggctga gcggacaaat cctctctgct ttttcgaaga tttctcttgg
      601 agctgcgaag acctctgaat ctccagaagt cagcacggtc tccgcctgct gtttttggaa
      661 gtgtatactg tacactggct agctgctcag ctctgttctt attagggatg atgcctcgtt
      721 ctacctcctt atgattttta ccaattcgaa tagcaagcca agagcccagt tttccattgt
      781 acaaggtatc cacaacacgg aacacctctc c
//
