LOCUS       sequence-96176-e        4497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yellow beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB775
FEATURES             Location/Qualifiers
     source          1..4497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(115..906,907..975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     738..757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(976..1067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1109..1128
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1134..1153
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1378..1398
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1483..1501)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(3614..3631)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3614..3630)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3729..3746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3900..4488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3980..3999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag
       61 ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gcttttacca
      121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc
      241 tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc
      301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
      361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      421 tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      961 atgttgaata ctcatattct tcctttttca atattattga agcatttatc agggttattg
     1021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt
     1081 tacaaccaat taaccaattc tgaacattat cgcgagccca tttatacctg aatatggctc
     1141 ataacacccc ttgtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     1201 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gactccccat gcgagagtag
     1261 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgcccg
     1321 ggctaattat ggggtgtcgc ccttcttagt cgctgaattt aattaaccat ggctccacca
     1381 aaaaaaaaaa gaaaagttgc catgggtggg ggcagcatgg aagacgccaa aaacataaag
     1441 aaaggcccgg cgccattcta tcctctagag gatggaaccg ctggagagca actgcataag
     1501 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
     1561 gtgaacatca cgtacgcgga atacttcgaa atgtccgttc ggttggcaga agctatgaaa
     1621 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     1681 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     1741 tataatgaac gtgaattgct caacagtatg aacatttcgc agcctaccgt agtgtttgtt
     1801 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat aatccagaaa
     1861 attattatca tggattctaa aacggattac cagggatttc agtcgatgta tacgttcgtc
     1921 acatctcatc tacctcccgg ttttaatgaa tacgattttg taccagagtc ctttgatcgt
     1981 gacaaaacaa ttgcactgat aatgaattcc tctggatcta ctgggttacc taagggtgtg
     2041 gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc
     2101 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
     2161 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
     2221 gaagaagagc tgtttttacg atcccttcag gattacaaaa ttcaaagtgc gttgctagta
     2281 ccaaccctat tttcattctt cgccaaaagc actctgattg acaaatacga tttatctaat
     2341 ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga agcggttgca
     2401 aaacgcttcc atcttccagg gatacgacaa ggatatgggc tcactgagac tacatcagct
     2461 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
     2521 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcagagaggc
     2581 gaattatgtg tcagaggacc tatgattatg tccggttatg taaacaatcc ggaagcgacc
     2641 aacgccttga ttgacaagga tggatggcta cattctggag accttgctta ctgggacgaa
     2701 gacgaacact tcttcatagt tgggcgcttg aagtctttaa tcaaatacaa aggttatcag
     2761 gtggcccccg ctgaattgga atcgatattg ttacaacacc ccaacatctt cgacgcgggc
     2821 gtggcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
     2881 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
     2941 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
     3001 aaaagagacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg aggtggcagt
     3061 atgtacaaaa ctagtgcagc gaacgacgaa aattacgccc ttgcagcgta ataactagtt
     3121 aatacaaata aggcgcgccc gtcaatcgag ttcgtaccta agggcgacac aaaatttatt
     3181 ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt gtattatcgt
     3241 tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc gagatttatt
     3301 ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga
     3361 tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc
     3421 gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc
     3481 gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg
     3541 aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg
     3601 gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa
     3661 aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct
     3721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct
     4321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcct
//
