LOCUS       sequence-96178-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses PDZ2 domain of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Fanning AS, Lye MF, Anderson JM, Lavie A
  TITLE     Domain swapping within PDZ2 is responsible for dimerization of ZO 
            proteins.
  JOURNAL   J Biol Chem. 2007 Dec 28;282(52):37710-6. Epub 2007 Oct 9.
  PUBMED    17928286
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-12-28"; volume: "282(52)"; pages: "37710-6. Epub
            2007 Oct 9"
COMMENT     Sequence Label: pET14b_tev_PDZ2
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(259..279)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(295..312)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             331..353
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(384..403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(385..403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(487..506)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtagcagccg gatcctcaat ctctttgaac taccattttt aatttgcctt tagacctttc
       61 tatcaatgtc tttgcatctg tcaatgacat attttctgtc acagtaccat ttatcttcaa
      121 tacaacatca ccttcttgaa tattgccatc tcttgctgcc aaactatctt gtgaaatttc
      181 cttaacaaat atatggcttg ccaatcgaag accatattct tcatttttcc gggatttcac
      241 cagtgtgact ttcatatggc cctgaaaata aagattctcg ttaccgccgc tgctgtgatg
      301 atgatgatga tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt
      361 ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga
      421 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      481 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgagctca
      541 tgagcgcttg tttcgacgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
      601 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
      661 tgtgctgctt cctaatg
//
