LOCUS       sequence-96179-e        4474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red beetle luciferase with nuclear localization signal (NLS) and 
            ClpXP degron tag (ssrA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB776
FEATURES             Location/Qualifiers
     source          1..4474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(112..903,904..972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     735..754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(973..1064)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1106..1125
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1131..1150
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1369..1389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3588..3605)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3588..3604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3703..3720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3874..4462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3954..3973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcttttcta cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga
       61 ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgct tttaccaatg
      121 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
      181 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
      241 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
      301 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      361 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      421 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      481 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      541 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
      601 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
      661 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
      721 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
      781 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
      841 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      901 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      961 ttgaatactc atattcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1021 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg tcagtgttac
     1081 aaccaattaa ccaattctga acattatcgc gagcccattt atacctgaat atggctcata
     1141 acaccccttg tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     1201 cagaagtgaa acgccgtagc gccgatggta gtgtggggac tccccatgcg agagtaggga
     1261 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgcccgggc
     1321 taattatggg gtgtcgccct tcgctgaatt taattaacca tggctccacc aaaaaaaaaa
     1381 agaaaagttg ccatgggtgg gggcagcatg gaagaagaaa acattgtgaa tggagatcgt
     1441 cctcgtgatc tagtttttcc tggcacagca ggactacaat tatatcaatc attatataaa
     1501 tattcatata ttactgacgg aataatcgat gcccatacca atgaagtaat atcatatgct
     1561 caaatatttg aaaccagctg ccgcttggca gttagtctag aaaaatatgg cttggatcat
     1621 aacaatgttg tggcaatatg cagtgaaaac aacatacact tttttggccc tttaattgct
     1681 gctttatacc aaggaatacc aatggcaaca tcaaatgata tgtatacaga aagggagatg
     1741 attggccatt tgaatatatc gaaaccatgc cttatgtttt gttcaaagaa atcactccca
     1801 tttattctga aagtacaaaa acatctagat ttccttaaaa aagtcatagt cattgatagt
     1861 atgtacgaca tcaatggcgt tgaatgcgta tttagctttg tttcacgtta tactgatcac
     1921 gcctttgatc cagtgaaatt taacccaaaa gagtttgatc ccttggaaag aaccgcatta
     1981 attatgacat catctggaac aactggattg cctaaagggg tagtaataag ccatagaagt
     2041 ataactataa gattcgtcca tagcagtgat cccatctatg gtactcgtat tgctccagat
     2101 acatcaattc ttgctatagc accgttccat catgcctttg gactgtttac tgcactagct
     2161 tactttccag taggacttaa gattgtaatg gtgaagaaat ttgagggcga attcttctta
     2221 aaaaccatac aaaattacaa aatcgcttct attgtagttc ctcctccaat tatggtatat
     2281 ttggctaaaa gtccattagt cgatgaatac aatttatcga gcttaacgga aattgcttgt
     2341 ggagggtctc ctttaggaag agacatcgca gataaagtag caaagagatt gaaagtacat
     2401 ggaatcctac aaggatatgg attaaccgaa acctgcagcg ctctaatact tagccccaat
     2461 gatcgagaac ttaaaaaagg tgcaattgga acgcctatgc catatgttca agttaaagtt
     2521 atagacatca atactgggaa ggcgctagga ccaagagaaa aaggcgaaat atgcttcaaa
     2581 agtcaaatgc ttatgaaagg ttatcacaac aatccgcaag caactcgtga tgctcttgac
     2641 aaagatggtt ggcttcatac tggggatctt ggatattacg acgaagacag atttatctat
     2701 gtagttgatc gattgaaaga acttattaaa tataaaggtt atcaggttgc gcctgctgaa
     2761 ctggaaaatc tgcttttaca acatccaaat atttctgatg cgggtgttat tggaattccg
     2821 gacgaatttg ctggtcaatt accttccgcg tgtgttgtgt tagagcctgg taagacaatg
     2881 accgaaaagg aagttcagga ttatattgca gagctagtca ctacaactaa acatcttcga
     2941 ggcggtgtcg tatttataga tagtattcca aaaggcccaa caggaaaact catgagaaac
     3001 gaactccgtg caatatttgc ccgggaacag gcaaaaggag gtggcagtat gtacaaaact
     3061 agtgcagcga acgacgaaaa ttacgccctt gcagcgtaat aactagttaa tacaaataag
     3121 gcgcgcccgt caaaagggcg acacaaaatt tattctaaat gcataataaa tactgataac
     3181 atcttatagt ttgtattata ttttgtatta tcgttgacat gtataatttt gatatcaaaa
     3241 actgattttc cctttattat tttcgagatt tattttctta attctcttta acaaactaga
     3301 aatattgtat atacaaaaaa tcataaataa tagatgaata gtttaattat aggtgttcat
     3361 caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct tttttctcat ttataaggtt
     3421 aaataattct catatatcaa gcaaagtgac aggcgccctt aaatattctg acaaatgctc
     3481 tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga
     3541 ttaacgatta ctcgttatca gaaccgccca gggggcccga gcttaagact ggccgtcgtt
     3601 ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct
     3661 tagtttgatg cctggcagtt ccctactctc gccttccgct tcctcgctca ctgactcgct
     3721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4261 aggcggtgct acagagttct tgaagtggtg ggctaactac ggctacacta gaagaacagt
     4321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4441 gcgcagaaaa aaaggatctc aagaagatcc tttg
//
