LOCUS       sequence-96185-e        4420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yellow beetle luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB687
FEATURES             Location/Qualifiers
     source          1..4420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(115..906,907..975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     738..757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(976..1067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1109..1128
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1134..1153
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1450..1468)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(3537..3554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3537..3553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3652..3669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3823..4411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3903..3922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac tcacgttaag
       61 ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gcttttacca
      121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc
      241 tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc
      301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
      361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      421 tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      961 atgttgaata ctcatattct tcctttttca atattattga agcatttatc agggttattg
     1021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt
     1081 tacaaccaat taaccaattc tgaacattat cgcgagccca tttatacctg aatatggctc
     1141 ataacacccc ttgtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     1201 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gactccccat gcgagagtag
     1261 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgcccg
     1321 ggctaattat ggggtgtcgc ccttcttagt cgctgaattt aattaaccat gggtgggggc
     1381 agcatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat
     1441 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     1501 attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg
     1561 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     1621 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     1681 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac
     1741 atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     1801 caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag
     1861 ggatttcagt cgatgtatac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     1921 gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct
     1981 ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc
     2041 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     2101 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     2161 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat
     2221 tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact
     2281 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt
     2341 tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga
     2401 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     2461 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     2521 aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc
     2581 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     2641 tctggagacc ttgcttactg ggacgaagac gaacacttct tcatagttgg gcgcttgaag
     2701 tctttaatca aatacaaagg ttatcaggtg gcccccgctg aattggaatc gatattgtta
     2761 caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa
     2821 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     2881 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     2941 gacgaagtac cgaaaggtct taccggaaaa agagacgcaa gaaaaatcag agagatcctc
     3001 ataaaggcca agaagggagg tggcagtatg tacaaaacta gttaatacaa ataaggcgcg
     3061 cccgtcaatc gagttcgtac ctaagggcga cacaaaattt attctaaatg cataataaat
     3121 actgataaca tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg
     3181 atatcaaaaa ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa
     3241 caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata
     3301 ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt
     3361 tataaggtta aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga
     3421 caaatgctct ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt
     3481 atttgcggat taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg
     3541 gccgtcgttt tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg
     3601 ccttctgctt agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac
     3661 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3721 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3781 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3841 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3901 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3961 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4021 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4081 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4141 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4201 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag
     4261 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4321 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4381 gcagattacg cgcagaaaaa aaggatctca agaagatcct
//
