LOCUS       sequence-96186-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SH3-GUK domains of human ZO-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Lye MF, Fanning AS, Su Y, Anderson JM, Lavie A
  TITLE     Insights into regulated ligand binding sites from the structure of 
            ZO-1 Src homology 3-guanylate kinase module.
  JOURNAL   J Biol Chem. 2010 Apr 30;285(18):13907-17. doi: 
            10.1074/jbc.M109.093674. Epub 2010 Mar 3.
  PUBMED    20200156
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.093674"; journalName: "J Biol Chem"; date: 
            "2010-04-30- 30"; volume: "285"; issue: "18"; pages: "13907-17"
COMMENT     Sequence Label: pET14b_SG
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             170..187
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             346..354
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gccggcccga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
      181 cggcagccat atgggagatt ctttctatat tagaacccat tttgaatatg aaaaggaatc
      241 tccctatgga cttagtttta acaaaggaga ggtgttccgt gttgtggata ccttgtacaa
      301 tggaaaactg ggctcttggc ttgctattcg aattggtaaa aatcataagg aggtagaacg
      361 aggcatcatc cctaataaga acagagctga gcagctagcc agtgtacagt atacacttcc
      421 aaaaacagca ggcggagacc gtgctgactt ctggagattc agaggtcttc gcagctccaa
      481 gagaaatctt cgaaaaagca gagaggattt gtccgctcag cctgttcaaa caaagtttcc
      541 agcttatgaa agagtggttc ttcgagaagc tggatttctg aggcctgtaa ccatttttgg
      601 accaatagct gatgttgcca gagaaaagct ggcaagagaa gaaccagata tttatcaaat
      661 tgcaaagagt gaaccacgag acgctggaac tgaccaacgt agctctggca ttattcgcct
      721 gcatacaata aagcaaatca tagatcaaga caaacatgct ttattagatg taacaccaaa
      781 tgcagttgat cgtcttaact atg
//
