LOCUS       sequence-96195-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses PDZ1 domain of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  TITLE     Plasmids for Structure of ZO-1 PDZ1 domain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET14b_tev_PDZ1
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             173..193
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(529..547)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      543..590
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccggcgttag aggatcgaga atctcgaatc ccgtcgtaaa ttaatacgac ttcacgtata
       61 gggagcacca ctacggattt cccgtctagc aaataatttt gtttaacctt taagaaggag
      121 atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcggta acgagaatct
      181 ttattttcag ggccatatga tatgggaaca acatacagtg acgcttcaca gggctcctgg
      241 atttggattt ggaattgcaa tatctggtgg acgagataat cctcattttc agagtgggga
      301 aacgtcaata gtgatttcag atgtgctgaa aggaggacca gctgaaggac agctacagga
      361 aaatgaccga gttgcaatgg ttaacggagt ttcaatggat aatgttgaac atgcttttgc
      421 tgttcagcaa ctaaggaaaa gtgggaaaaa tgcaaaaatt acaattagaa ggaagtgagg
      481 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      541 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      601 gaactatttc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
      661 gctccaagta gcgaagcgag caggactggg cggcggcc
//
