LOCUS       sequence-96196-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses PDZ1 domain of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     Plasmids for Structure of ZO-1 PDZ1 domain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET14b_tev_PDZ1
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(302..322)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(338..355)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             374..396
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(427..446)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(428..446)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(530..549)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttagcagcc ggatcctcac ttccttctaa ttgtaatttt tgcatttttc ccacttttcc
       61 ttagttgctg aacagcaaaa gcatgttcaa cattatccat tgaaactccg ttaaccattg
      121 caactcggtc attttcctgt agctgtcctt cagctggtcc tcctttcagc acatctgaaa
      181 tcactattga cgtttcccca ctctgaaaat gaggattatc tcgtccacca gatattgcaa
      241 ttccaaatcc aaatccagga gccctgtgaa gcgtcactgt atgttgttcc catatcatat
      301 ggccctgaaa ataaagattc tcgttaccgc cgctgctgtg atgatgatga tgatggctgc
      361 tgcccatggt atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg
      421 tggtctccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
      481 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
      541 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
      601 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc nttgcatgca
      661 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactggggct gcttcctaat
      721 gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttc
//
