LOCUS       sequence-96211-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Rab5 fused to mRFP. Used for localization to
            the early endosome, and other early endosomal vacuoles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Vonderheit A, Helenius A
  TITLE     Rab7 associates with early endosomes to mediate sorting and 
            transport of Semliki forest virus to late endosomes.
  JOURNAL   PLoS Biol. 2005 Jul . 3(7):e233.
  PUBMED    15954801
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2005 Jul . 3(7):e233."
COMMENT     Sequence Label: mRFP-Rab5
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..714
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             40..714
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(170..188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     438..457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     632..655
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggcctcctc cgaggacgtc
       61 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
      121 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      181 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttccagtac
      241 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      301 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      361 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      421 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
      481 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg
      541 aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg
      601 cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac
      661 tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgccctgtac
      721 aagtactcag atctcgagct caagcttcga attcc
//
