LOCUS       sequence-96225-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Magnetic cell sorting of ES cell-derived astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, 
            Burrone J, Greensmith L, Lieberam I
  TITLE     Optical control of muscle function by transplantation of stem 
            cell-derived motor neurons in mice.
  JOURNAL   Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.
  PUBMED    24700859
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-4- 4"; volume: "344"; issue: 
            "6179"; pages: "94-7"
COMMENT     Sequence Label: tol2-GFAP::CD14
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(269..288)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    313..360
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    365..412
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(432..451)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    497..552
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(551..570)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
       61 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
      121 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
      181 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
      241 tcttcgccca ccctaggggg aggctgactg aaacacggaa ggagacaata ccggaaggaa
      301 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
      361 ataagaagtt cctattccga agttcctatt ctctagaaag tataggaact tcgtcgagtc
      421 tagatatcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
      481 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
      541 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
      601 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
      661 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
      721 aaagccttga cttgaggtta gattttt
//
