LOCUS       sequence-96248-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for Drosophila melanogaster Or24a expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Vosshall LB, Amrein H, Morozov PS, Rzhetsky A, Axel R
  TITLE     A spatial map of olfactory receptor expression in the Drosophila 
            antenna.
  JOURNAL   Cell. 1999 Mar 5;96(5):725-36.
  PUBMED    10089887
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1999-03-5"; volume: "96(5)"; pages: "725-36"
COMMENT     Sequence Label: pGEM-T Dmel Or24a
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(901..919)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(902..919)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(937..953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(937..953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(950..972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    961..977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(985..991,992..1009,1010..1015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1030..1051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg
       61 gccgcgggat tacatagcca ctgcattgga tgccctttgt cctgtggcct ccagcatttt
      121 gtcgctggtg aaaatggtcg ctatttggtg gtatcaagat gaattaagga gtttgataga
      181 gcgggtgaga tttttaacag agcaacagag gtccaagagg aaactgggct ataagaagag
      241 gttctataca ctggcaacgc aactaacatt cctgctacta tgctgtggat tttgcaccag
      301 tacttcctat tccgtcagac atttgattga taatatcctg agacgcaccc atggcaagga
      361 ctggatctac gagaccccgt tcaagatgat gtaaggaaag ggaagaatgg tttatatata
      421 cttttggaac gaaataatga tgtgatctaa acaagatgca cttttaggtt ccccgatcct
      481 ctcctgcgtt tgccactcta tcccatcacc tatatactcg tgcattggca tggctacatt
      541 actgtggttt gttttgtcgg cgcggatggt ttcttcctgg ggttctgttt gtacttcact
      601 gttttgctgc tctgtctgca ggacgatgtt ggtgatttac tagaggttga aaacatcgaa
      661 aagagtccct ccgaagcgga ggaagctcgc atagttcggg aaatggaaaa actggtggac
      721 cggcataacg aggtggccga gctgacagaa agattgtcgg gtgttatggt ggaaataaca
      781 ctggcccact ttgttacttc gagtttgata atcggaacca gcgtggaatc actagtgcgg
      841 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat
      901 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      961 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1021 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctt
//
