LOCUS       sequence-9626-ex        9209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Sorg G, Stamminger T
  TITLE     Mapping of nuclear localization signals by simultaneous fusion to 
            green fluorescent protein and to beta-galactosidase.
  JOURNAL   Biotechniques. 1999 May . 26(5):858-62.
  PUBMED    10337476
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 1999 May . 26(5):858-62."
COMMENT     Sequence Label: pHM829
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             895..3939
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(897..919)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(4761..4779)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4762..4779)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4799..4816)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4805..5029
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5075..5503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5162..5181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5372..5393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5512..5532)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5517..5847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5698..5833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5760..5779
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5914..6708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5968..5987)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6578..6597
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6882..7003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6919..6938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6973..6992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7052..7068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7052..7068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7065..7087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7076..7092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7100..7106,7107..7124,7125..7130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7145..7166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7283..7300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7454..8042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7534..7553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8213..9004,9005..9073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8836..8855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9074..9178)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgtcgtt
      901 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      961 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1021 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     1081 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     1141 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
     1201 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     1261 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
     1321 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
     1381 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
     1441 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
     1501 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
     1561 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
     1621 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
     1681 accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
     1741 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
     1801 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
     1861 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
     1921 attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
     1981 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
     2041 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
     2101 gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
     2161 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
     2221 ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
     2281 ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
     2341 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
     2401 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
     2461 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
     2521 aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
     2581 gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
     2641 gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
     2701 cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
     2761 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
     2821 ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
     2881 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
     2941 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
     3001 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
     3061 cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
     3121 ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
     3181 ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
     3241 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
     3301 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
     3361 gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
     3421 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
     3481 gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
     3541 gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
     3601 actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
     3661 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
     3721 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
     3781 agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
     3841 atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc
     3901 gccggtcgct accattacca gttggtctgg tgtcaaaagc taggcccggg cccgctagcg
     3961 gccttaaggg cccgcggggc cacgtcggct ctagagggcc cgggcctaga gctctcgagg
     4021 ctggtaccat gagcaagggc gaggaactgt tcactggcgt ggtcccaatt ctcgtggaac
     4081 tggatggcga tgtgaatggg cacaaatttt ctgtcagcgg agagggtgaa ggtgatgcca
     4141 catacggaaa gctcaccctg aaattcatct gcaccactgg aaagctccct gtgccatggc
     4201 caacactggt cactaccttc tcttatggcg tgcagtgctt ttccagatac ccagaccata
     4261 tgaagcagca tgactttttc aagagcgcca tgcccgaggg ctatgtgcag gagagaacca
     4321 tctttttcaa agatgacggg aactacaaga cccgcgctga agtcaagttc gaaggtgaca
     4381 ccctggtgaa tagaatcgag ctgaagggca ttgactttaa ggaggatgga aacattctcg
     4441 gccacaagct ggaatacaac tataactccc acaatgtgta catcatggcc gacaagcaaa
     4501 agaatggcat caaggtcaac ttcaagatca gacacaacat tgaggatgga tccgtgcagc
     4561 tggccgacca ttatcaacag aacactccaa tcggcgacgg ccctgtgctc ctcccagaca
     4621 accattacct gtccacccag tctgccctgt ctaaagatcc caacgaaaag agagaccaca
     4681 tggtcctgct ggagtttgtg accgctgctg ggatcacaca tggcatggac gagctgtaca
     4741 agtgagaatt cgggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     4801 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4861 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4921 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4981 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5041 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5221 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5281 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5401 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     5461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5521 gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg
     5581 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5641 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     5701 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5761 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5821 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5881 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5941 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6001 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6061 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     6121 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6181 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6241 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6301 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6361 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6421 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     6481 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6541 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6601 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6661 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6721 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6781 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6841 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7021 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7081 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7141 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7201 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7261 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7321 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7381 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7441 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7501 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7561 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7621 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     7681 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7741 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7801 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7861 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7921 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7981 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8041 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8101 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8161 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8221 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8281 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8341 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8401 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8461 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8521 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8581 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8641 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8701 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8761 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8821 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8881 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8941 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9001 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9061 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9121 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9181 catttccccg aaaagtgcca cctgacgtc
//
