LOCUS       sequence-96262-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet on shRNA for conditional Yap1 knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel 
            AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks
            T, Hahn WC
  TITLE     KRAS and YAP1 Converge to Regulate EMT and Tumor Survival.
  JOURNAL   Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. 
            Epub 2014 Jun 19.
  PUBMED    24954536
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06.004"; journalName: "Cell"; date: "2014-07-3-
            3"; volume: "158"; issue: "1"; pages: "171-84"
COMMENT     Sequence Label: TRMPVneo.Yap.3093
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(38..164)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(192..206)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(234..355)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(486..509)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcgcctcccc tacccggtag aattgctaga attgaaaaaa gtgatttaat ttataccatt
       61 ttaattcagc tttgtaaaaa tgtatcaaag agatagcaag gtattcagtt ttagtaaaca
      121 agataattgc tcctaaagta gccccttgaa ttccgaggca gtaggcacat gaattaactc
      181 tgtgtataat acatctgtgg cttcactatt atacacagag ttaattcatt cgctcactgt
      241 caacagcaat ataccttctc gagccttctg ttgggttaac ctgaagaagt aatcccagca
      301 agtgtttcca agatgtgcag gcaacgattc tgtaaagtac tgaagcctca ttcaaacaat
      361 taccctgtta tccctagtcg agagatctta tcatatgact agtaaattct agagtcgcgg
      421 ccgctacagg aacaggtggt ggcggccctc ggtgcgctcg tactgctcca cgatggtgta
      481 gtcctcgttg tgggaggtga tgtccagctt ggcgtccacg tagtagtagc cgggcagctg
      541 cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc cgccgtcctt
      601 cagcttcagg gccttgtggg tctcgccctt cagcacgccg tcgcgggggt acaggcgctc
      661 ggtggaggcc tcccagccca tggtcttctt ctgcatcacg gggccgtcgg aggggaagtt
      721 cacgccgatg aacttcacct tgta
//
