LOCUS       sequence-96286-e        8572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains human dysferlin with exon 37 deleted. Deletion 
            maintains the open reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8572)
  TITLE     Human dysferlin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: DYSF-Exon37-del
FEATURES             Location/Qualifiers
     source          1..8572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             897..908
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      6387..6396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6396..6412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6396..6412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6409..6431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6420..6436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6444..6450,6451..6468,6469..6474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6489..6510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6627..6644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6798..7386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6878..6897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7557..8372)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7557..8372)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8280..8299
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8373..8477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8545..8563
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 acgaattcga tatcgttaac ggtaccacta gtacgcgtat gctgagggtc ttcatcctct
      241 atgccgagaa cgtccacaca cccgacaccg acatcagcga tgcctactgc tccgcggtgt
      301 ttgcaggggt gaagaagaga accaaagtca tcaagaacag cgtgaaccct gtatggaatg
      361 agggatttga atgggacctc aagggcatcc ccctggacca gggctctgag cttcatgtgg
      421 tggtcaaaga ccatgagacg atggggagga acaggttcct gggggaagcc aaggtcccac
      481 tccgagaggt cctcgccacc cctagtctgt ccgccagctt caatgccccc ctgctggaca
      541 ccaagaagca gcccacaggg gcctcgctgg tcctgcaggt gtcctacaca ccgctgcctg
      601 gagctgtgcc cctgttcccg ccccctactc ctctggagcc ctccccgact ctgcctgacc
      661 tggatgtagt ggcagacaca ggaggagagg aagacacaga ggaccaggga ctcactggag
      721 atgaggcgga gccattcctg gatcaaagcg gaggcccggg ggctcccacc accccaagga
      781 aactaccttc acgtcctccg ccccactacc ccgggatcaa aagaaagcga agtgcgccta
      841 catctagaaa gctgctgtca gacaaaccgc aggatttcca gatcagggtc caggtgatcg
      901 aggggcgcca gctgccgggg gtgaacatca agcctgtggt caaggttacc gctgcagggc
      961 agaccaagcg gacgcggatc cacaagggaa acagcccact cttcaatgag actcttttct
     1021 tcaacttgtt tgactctcct ggggagctgt ttgatgagcc catctttatc acggtggtag
     1081 actctcgttc tctcaggaca gatgctctcc tcggggagtt ccggatggac gtgggcacca
     1141 tttacagaga gccccggcac gcctatctca ggaagtggct gctgctctca gaccctgatg
     1201 acttctctgc tggggccaga ggctacctga aaacaagcct ttgtgtgctg gggcctgggg
     1261 acgaagcgcc tctggagaga aaagacccct ctgaagacaa ggaggacatt gaaagcaacc
     1321 tgctccggcc cacaggcgta gccctgcgag gagcccactt ctgcctgaag gtcttccggg
     1381 ccgaggactt gccgcagatg gacgatgccg tgatggacaa cgtgaaacag atctttggct
     1441 tcgagagtaa caagaagaac ttggtggacc cctttgtgga ggtcagcttt gcggggaaaa
     1501 tgctgtgcag caagatcttg gagaagacgg ccaaccctca gtggaaccag aacatcacac
     1561 tgcctgccat gtttccctcc atgtgcgaaa aaatgaggat tcgtatcata gactgggacc
     1621 gcctgactca caatgacatc gtggctacca cctacctgag tatgtcgaaa atctctgccc
     1681 ctggaggaga aatagaagag gagcctgcag gtgctgtcaa gccttcgaaa gcctcagact
     1741 tggatgacta cctgggcttc ctccccactt ttgggccctg ctacatcaac ctctatggca
     1801 gtcccagaga gttcacaggc ttcccagacc cctacacaga gctcaacaca ggcaaggggg
     1861 aaggtgtggc ttatcgtggc cggcttctgc tctccctgga gaccaagctg gtggagcaca
     1921 gtgaacagaa ggtggaggac cttcctgcgg atgacatcct ccgggtggag aagtacctta
     1981 ggaggcgcaa gtactccctg tttgcggcct tctactcagc caccatgctg caggatgtgg
     2041 atgatgccat ccagtttgag gtcagcatcg ggaactacgg gaacaagttc gacatgacct
     2101 gcctgccgct ggcctccacc actcagtaca gccgtgcagt ctttgacggg tgccactact
     2161 actacctacc ctggggtaac gtgaaacctg tggtggtgct gtcatcctac tgggaggaca
     2221 tcagccatag aatcgagact cagaaccagc tgcttgggat tgctgaccgg ctggaagctg
     2281 gcctggagca ggtccacctg gccctgaagg cgcagtgctc cacggaggac gtggactcgc
     2341 tggtggctca gctgacggat gagctcatcg caggctgcag ccagcctctg ggtgacatcc
     2401 atgagacacc ctctgccacc cacctggacc agtacctgta ccagctgcgc acccatcacc
     2461 tgagccaaat cactgaggct gccctggccc tgaagctcgg ccacagtgag ctccctgcag
     2521 ctctggagca ggcggaggac tggctcctgc gtctgcgtgc cctggcagag gagccccaga
     2581 acagcctgcc ggacatcgtc atctggatgc tgcagggaga caagcgtgtg gcataccagc
     2641 gggtgcccgc ccaccaagtc ctcttctccc ggcggggtgc caactactgt ggcaagaatt
     2701 gtgggaagct acagacaatc tttctgaaat atccgatgga gaaggtgcct ggcgcccgga
     2761 tgccagtgca gatacgggtc aagctgtggt ttgggctctc tgtggatgag aaggagttca
     2821 accagtttgc tgaggggaag ctgtctgtct ttgctgaaac ctatgagaac gagactaagt
     2881 tggcccttgt tgggaactgg ggcacaacgg gcctcaccta ccccaagttt tctgacgtca
     2941 cgggcaagat caagctaccc aaggacagct tccgcccctc ggccggctgg acctgggctg
     3001 gagattggtt cgtgtgtccg gagaagactc tgctccatga catggacgcc ggtcacctga
     3061 gcttcgtgga agaggtgttt gagaaccaga cccggcttcc cggaggccag tggatctaca
     3121 tgagtgacaa ctacaccgat gtgaacgggg agaaggtgct tcccaaggat gacattgagt
     3181 gcccactggg ctggaagtgg gaagatgagg aatggtccac agacctcaac cgggctgtcg
     3241 atgagcaagg ctgggagtat agcatcacca tccccccgga gcggaagccg aagcactggg
     3301 tccctgctga gaagatgtac tacacacacc gacggcggcg ctgggtgcgc ctgcgcagga
     3361 gggatctcag ccaaatggaa gcactgaaaa ggcacaggca ggcggaggcg gagggcgagg
     3421 gctgggagta cgcctctctt tttggctgga agttccacct cgagtaccgc aagacagatg
     3481 ccttccgccg ccgccgctgg cgccgtcgca tggagccact ggagaagacg gggcctgcag
     3541 ctgtgtttgc ccttgagggg gccctgggcg gcgtgatgga tgacaagagt gaagattcca
     3601 tgtccgtctc caccttgagc ttcggtgtga acagacccac gatttcctgc atattcgact
     3661 atgggaaccg ctaccatcta cgctgctaca tgtaccaggc ccgggacctg gctgcgatgg
     3721 acaaggactc tttttctgat ccctatgcca tcgtctcctt cctgcaccag agccagaaga
     3781 cggtggtggt gaagaacacc cttaacccca cctgggacca gacgctcatc ttctacgaga
     3841 tcgagatctt tggcgagccg gccacagttg ctgagcaacc gcccagcatt gtggtggagc
     3901 tgtacgacca tgacacttat ggtgcagacg agtttatggg tcgctgcatc tgtcaaccga
     3961 gtctggaacg gatgccacgg ctggcctggt tcccactgac gaggggcagc cagccgtcgg
     4021 gggagctgct ggcctctttt gagctcatcc agagagagaa gccggccatc caccatattc
     4081 ctggttttga ggtgcaggag acatcaagga tcctggatga gatcctggca tggggcctgc
     4141 ggaacatgaa gagttaccag ctggccaaca tctcctcccc cagcctcgtg gtagagtgtg
     4201 ggggccagac ggtgcagtcc tgtgtcatca ggaacctccg gaagaacccc aactttgaca
     4261 tctgcaccct cttcatggaa gtgatgctgc ccagggagga gctctactgc ccccccatca
     4321 ccgtcaaggt catcgataac cgccagtttg gccgccggcc tgtggtgggc cagtgtacca
     4381 tccgctccct ggagagcttc ctgtgtgacc cctactcggc ggagagtcca tccccacagg
     4441 gtggcccaga cgatgtgagc ctactcagtc ctggggaaga cgtgctcatc gacattgatg
     4501 acaaggagcc cctcatcccc atccaggagg aagagttcat cgattggtgg agcaaattct
     4561 ttgcctccat aggggagagg gaaaagtgcg gctcctacct ggagaaggat tttgacaccc
     4621 tgaaggtcta tgacacacag ctggagaatg tggaggcctt tgagggcctg tctgactttt
     4681 gtaacacctt caagctgtac cggggcaaga cgcaggagga gacagaagat ccatctgtga
     4741 ttggtgaatt taagggcctc ttcaaaattt atcccctccc agaagaccca gccatcccca
     4801 tgcccccaag acagttccac cagctggccg cccagggacc ccaggagtgc ttggtccgta
     4861 tctacattgt ccgagcattt ggcctgcagc ccaaggaccc caatggaaag tgtgatcctt
     4921 acatcaagat ctccataggg aagaaatcag tgagtgacca ggataactac atcccctgca
     4981 cgctggagcc cgtatttgga aagatgttcg agctgacctg cactctgcct ctggagaagg
     5041 acctaaagat cactctctat gactatgacc tcctctccaa ggacgaaaag atcggtgaga
     5101 cggtcgtcga cctggagaac aggctgctgt ccaagtttgg ggctcgctgt ggactcccac
     5161 agacctactg tgtctctgga ccgaaccagt ggcgggacca gctccgcccc tcccagctcc
     5221 tccacctctt ctgccagcag catagagtca aggcacctgt gtaccggaca gaccgtgtaa
     5281 tgtttcagga taaagaatat tccattgaag agatagaggc tggcaggatc ccaaacccac
     5341 acctgggccc agtggaggag cgtctggctc tgcatgtgct tcagcagcag ggcctggtcc
     5401 cggagcacgt ggagtcacgg cccctctaca gccccctgca gccagacatc gagcagggga
     5461 agctgcagat gtgggtcgac ctatttccga aggccctggg gcggcctgga cctcccttca
     5521 acatcacccc acggagagcc agaaggtttt tcctgcgttg tattatctgg aataccagag
     5581 atgtgatcct ggatgacctg agcctcacgg gggagaagat gagcgacatt tatgtgaaag
     5641 gttggatgat tggctttgaa gaacacaagc aaaagacaga cgtgcattat cgttccctgg
     5701 gaggtgaagg caacttcaac tggaggttca ttttcccctt cgactacctg ccagctgagc
     5761 aagtctgtac cattgccaag aaggatgcct tctggaggct ggacaagact gagagcaaaa
     5821 tcccagcacg agtggtgttc cagatctggg acaatgacaa gttctccttt gatgattttc
     5881 tgggctccct gcagctcgat ctcaaccgca tgcccaagcc agccaagaca gccaagaagt
     5941 gctccttgga ccagctggat gatgctttcc acccagaatg gtttgtgtcc ctttttgagc
     6001 agaaaacagt gaagggctgg tggccctgtg tagcagaaga gggtgagaag aaaatactgg
     6061 cgggcaagct ggaaatgacc ttggagattg tagcagagag tgagcatgag gagcggcctg
     6121 ctggccaggg ccgggatgag cccaacatga accctaagct tgaggaccca aggcgccccg
     6181 acacctcctt cctgtggttt acctccccat acaagaccat gaagttcatc ctgtggcggc
     6241 gtttccggtg ggccatcatc ctcttcatca tcctcttcat cctgctgctg ttcctggcca
     6301 tcttcatcta cgccttcccg aactatgctg ccatgaagct ggtgaagccc ttcagctgat
     6361 gatcaccatg gcatatggct agcgcggccg ccatggtcat agctgtttcc tgtgtgaaat
     6421 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6481 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6541 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttag
     7561 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     7621 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     7681 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     7741 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     7801 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     7861 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     7921 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     7981 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     8041 tctaatacct ggaatgctgt tttcccaggg atcgcagtgg tgagtaacca tgcatcatca
     8101 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     8161 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     8221 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     8281 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     8341 gagcaagacg tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc
     8401 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8461 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8521 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
