LOCUS       sequence-96292-e        9534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse NLRC4 expressed under the constitutive, moderate-level 
            retroviral LTR promoter, expresses in mammalian cells; IRES-GFP 
            reporter downstream of NLRC4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-NLRC4
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3269..3835
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3285..3837
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3429..3446)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3656..3675
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3849..3851,3852..3854,3855..4568)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3894..3915)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4155..4174)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4502..4523
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4621..5135
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5304..5320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5317..5339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5328..5344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5352..5358,5359..5376,5377..5382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5397..5418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5535..5552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5706..6294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5786..5805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6465..7256,7257..7325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7088..7107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7326..7430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7498..7516
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7554..7576)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7676..7695
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7889..7911
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8039..8058
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8258..8774
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    8838..9179
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(9246..9534,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatcc gccaccatga actttataag gaacaacaga cgagccctta
      181 ttcaaaggat gggcttaaca gttaccaagc aaatctgcga tgacctcttt gcattgaacg
      241 ttctcaacaa tcaagaagct aatgtcattt actgtgagcc cttggagcag gaagccgccc
      301 gaaagatcat ccatatgact atgcagaagg gctcagcggc ctgcaacctc tttcttaaga
      361 gtcttgaaaa ctgggactat tttgtgtatc aggacttaac tggacaaaat ctttcttatc
      421 aggtcacaga agaagacctg aatgttttgg cccagaattt aaaggacttg tacaacagcc
      481 ctgcttttct gaacttctac cccctgggtg aagatatcga cataattttt aatctggaga
      541 aaaccttcac agaacctatc atgtggaaga aggaccatcg tcatcaccgt gtggagcagc
      601 tgactttggg cagcctgctc gaggctctga agagcccctg cctgattgaa ggcgagtctg
      661 gcaaagggaa gtccaccctg ctgcagagaa tcgctatgct ctgggcctct gggggctgca
      721 gggctctgaa ggggttcaga ttagtcttct tcatccacct gagaagcgcc agggggggac
      781 tattcgaaac actgtacgat cagctcctga acatacccga cttcatcagc aagccgacct
      841 tcaaggctct gctgctgaag ctacacaagg aggtcctctt tcttctcgat ggttacaatg
      901 aattccatcc ccagaactgc ccagaaattg aagccctgat aaaggaaaac catcgcttca
      961 agaacatggt cattgtcacc accaccacgg agtgcctgag gcatatcaga catgttggcg
     1021 ccctgactgc ggaggtggga gatatgaccg aagacagtgc caaagatctc atcgaggcag
     1081 tgctggtacc tgatcaggtt gaacgcctgt gggcccaaat ccaggagtcc aggtgcctga
     1141 gaaatctgat gaagacccct ctcttcgtgg tgatcacctg tgcaattcag atgggcagac
     1201 aggaattcca agctcacacc caaaccatgc tgttccaaac cttctacgac ctcctgatac
     1261 agaaaaacag ccacagatat agaggtggag cttcaggtga ttttgccagg agcctagact
     1321 actgtggaga cctggcccta gaaggtgtgt tcgcccacaa atttgatttt gaacccgagc
     1381 atgggtccag catgaacgag gacgtcctgg tgacaatagg gctcctctgt aagtacacag
     1441 ctcagaggct gaagcccacg tataaattct ttcataaatc atttcaggag tacacggcag
     1501 gtcggagact cagcagtttg ctgacgtcca aagagccaga ggaggtgagc aaagggaaca
     1561 gctacttaaa caaaatggtt tccatctctg acatcacatc cctatatggc aatctgctcc
     1621 tctacacgtg tgggtcgtcc acagaagcaa ccagggcggt catgaggcac cttgcaatgg
     1681 tttatcagca cggcagccta caaggacttt cagtcaccaa gaggcctctc tggaggcagg
     1741 aatcaatcca gagtctgaga aataccactg agcaagatgt tctgaaagcc atcaatgtaa
     1801 attccttcgt agagtgtggc atcaatttgt tctcagagag tatgtctaaa tcagacctga
     1861 gccaagaatt tgaagctttc tttcaaggta aaagtttata catcaactca gagaacatcc
     1921 ctgactattt atttgacttc tttgaatact tgcctaattg tgcaagcgca ttggacttcg
     1981 tgaagttgga tttctatgaa agagctacag agtcacagga caaggcagaa gagaatgtcc
     2041 ctggagttca cacagaaggg ccctcagaaa cctacattcc ccccagggct gtgtctttgt
     2101 tcttcaactg gaagcaggaa ttcaagactc tagaggtcac actccgagat attaacaagt
     2161 tgaataagca agatatcaaa tatctgggga agatattcag ctctgccacc aacctccggc
     2221 tgcatatcaa gagatgtgca gccatggctg gaagactcag ctcagtcctc agaacctgca
     2281 agaacatgca taccctcatg gtggaagcca gtcccctcac cacggatgac gaacagtaca
     2341 tcacatctgt gacaggcctc cagaacttaa gtattcaccg cttgcacact caacagctgc
     2401 caggtggtct gattgacagc ttgggtaatc tgaagaacct cgagagactc atactggatg
     2461 acatcaggat gaacgaggaa gatgctaaaa acctagccga aggcctacga agcctgaaga
     2521 agatgcgttt actccatttg actcatttgt ctgacattgg ggaggggatg gactacatag
     2581 tcaagtctct ctcagaagaa tcctgtgatc tccaagagat gaagttggtg gcctgctgtc
     2641 tgactgcaaa ctctgtgaaa gttctagcac agaatcttca caatttgatc aagctgagca
     2701 ttcttgatat atcagaaaat tacctggaaa aggatgggaa tgaagctcta caggaactga
     2761 tcggcaggct tggcgttctg ggagagctca ctacattgat gctgccttgg tgctgggatg
     2821 tgcacaccag cctgcccaag ctgttgaagc agttggaggg gaccccagga cttgccaaac
     2881 ttggattgaa aaactggaga ctcagagacg aagagattaa aagtttaggt gaatttctgg
     2941 agatgaatcc tctgagagac ttgcagcagt tggatttagc ggggcactgt gtgagcagtg
     3001 acggatggct ttacttcatg aatgtgtttg agaatctgaa gcagttagtg ttttttgact
     3061 ttagcactga ggagttctta ccggatgcag cactggtgag gaaacttagt caagtgttat
     3121 ccaagttaac tcttctgcaa gaggtaaagc tcacgggctg ggagtttgat gactatgata
     3181 ttagcgctat taaaggcacc tttaaactag tgactgctta agcggccgcg tttaaacgtc
     3241 gacggtatcg ataagcttga tatcgaattc cgcccccccc cctaacgtta ctggccgaag
     3301 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3361 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3421 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3481 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3541 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     3601 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     3661 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     3721 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     3781 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     3841 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3901 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg
     3961 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4021 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4081 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4141 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4201 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4261 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     4321 agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4381 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4441 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4501 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4561 acaagtgata agaattcgat aaaataaaag attttattta gtctccagaa aaagggggga
     4621 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     4681 tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc
     4741 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgcccccggc tcagggccaa
     4801 gaacagatgg tccccgatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt
     4861 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     4921 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     4981 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat
     5041 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     5101 gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt gaagttggag
     5161 aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt agccactgtt
     5221 ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc agaagagctg
     5281 gggagaatta attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6121 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7441 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7501 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     7561 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7621 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     7681 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     7741 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     7801 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     7861 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa
     7921 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     7981 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     8041 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     8101 tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg
     8161 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     8221 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     8281 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     8341 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
     8401 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     8461 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     8521 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     8581 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     8641 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc
     8701 cgaatcgtgg actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc
     8761 gggggtcttt catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga
     8821 cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc
     8881 gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct
     8941 gtatctggcg gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga
     9001 gacgtcccag ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt
     9061 ggaatccgac cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc
     9121 cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc
     9181 agcgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa
     9241 attagggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     9301 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     9361 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     9421 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     9481 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtca
//
