LOCUS       sequence-96299-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of human FOXH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMKO.1-puro/hFOXH1 shRNA6
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        74..322
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     74..94
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     245..264
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(407..427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        412..741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      592..727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(751..770)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atctccttta tccagccctc actccttctc taggcgccgg aattgaagat ctgtcgacaa
       61 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
      121 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
      181 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      241 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      301 cttgtggaaa ggacgaaaca ccggtgcagc ctgtgaggct cttaactcga gttacgagcc
      361 tcacaggctg catttttgaa ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag
      421 ttagggtgtg gaaagtcccc aggcttccca gcaggcagaa gtatgcaaag catgcatctc
      481 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      541 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      601 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      661 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      721 ggaggcctag gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc
      781 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
      841 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
      901 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
      961 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     1021 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     1081 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcgg
//
