LOCUS       sequence-96301-e        1242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- 
            Cre(60-343).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1242)
  TITLE     Herman's lab DiCre2-59.F2 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA_DiCre2_60.F2
FEATURES             Location/Qualifiers
     source          1..1242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             34..354
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atggccccta agaagaagag aaaggtgtct agaggagtgc aggtggaaac catctcccca
       61 ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta caccgggatg
      121 cttgaagatg gaaagaaagt tgattcctcc cgggacagaa acaagccctt taagtttatg
      181 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt
      241 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc
      301 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact ggaagctagc
      361 cccagcaacc ccggcgccag caacggatcc aacaggaaat ggttccctgc tgaacctgag
      421 gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa
      481 cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac
      541 tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag
      601 agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg
      661 gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac
      721 accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat
      781 ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg
      841 gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt
      901 gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc
      961 ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac
     1021 cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac
     1081 tctgccagag tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc
     1141 atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag aaacctggac
     1201 tctgagactg gggccatggt gaggctgctc gaggatgggg ac
//
