LOCUS       sequence-96303-e        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Jullien N, Sampieri F, Enjalbert A, Herman JP
  TITLE     Regulation of Cre recombinase by ligand-induced complementation of 
            inactive fragments.
  JOURNAL   Nucleic Acids Res. 2003 Nov 1;31(21):e131.
  PUBMED    14576331
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2003-11-1"; volume: "31(21)"; pages: "e131"
COMMENT     Sequence Label: pcDNA_DiCre_60.F2
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             34..309
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
ORIGIN
        1 atggccccta agaagaagag aaaggtgtct agaatcctct ggcatgagat gtggcatgaa
       61 ggcctggaag aggcatctcg tttgtacttt ggggaaagga acgtgaaagg catgtttgag
      121 gtgctggagc ccttgcatgc tatgatggaa cggggccccc agactctgaa ggaaacatcc
      181 tttaatcagg cctatggtcg agatttaatg gaggcccaag agtggtgcag gaagtacatg
      241 aaatcaggga atgtcaagga cctcctccaa gcctgggacc tctattatca tgtgttccga
      301 cgaatctcag ctagccccag caaccccggc gccagcaacg gatccaacag gaaatggttc
      361 cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag aggcctggct
      421 gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag atctggcctg
      481 cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat
      541 gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga ctttgaccaa
      601 gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct ggccttcctg
      661 ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag agtgaaggac
      721 atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa gaccctggtg
      781 tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt ggagagatgg
      841 atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg ggtcagaaag
      901 aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct ggaagggatc
      961 tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca gagatacctg
     1021 gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag ggctggtgtg
     1081 tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt gatgaactac
     1141 atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga tggggac
//
