LOCUS       sequence-96316-e        8917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in 
            pDONR221 plasmid which can be used as an entry vector for 
            Invitrogen's gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8917)
  TITLE     Mouse dysferlin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Mouse-Dysferlin-Transcript-Variant1
FEATURES             Location/Qualifiers
     source          1..8917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1484..1495
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7042..7141)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7158..7177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7159..7177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7182..7198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7182..7198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7300..7319)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7311..8120
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7378..7397)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8267..8855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8756..8775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctca ctagtggtac cgtttaaacg atcggccgcc accatgctct gttgcttgct
      721 ggcaagggcc agcaacctcc ccaatgtgaa gaaggaccgg cgcagcgatc cagttgccag
      781 cctgattttt cgaggggtaa agaagagaac caaagtcatc aagaacagtg tgaaccccgt
      841 gtggaatgag ggctttgagt gggacctcaa aggtattcct ctagatcaga gctcagaact
      901 tctcgtggtg gtcaaggacc atgagacgat gggaagaaac aggttcctgg gggaagccaa
      961 gatcccactc caggaggtcc ttgccacccc cagcctctct gccagcttca acgcacctct
     1021 gctagacgcc aagcagcaac ccacgggggc ctctctggtc ctgcaggtgt cctacacgcc
     1081 acccccagga gctgtgcccc tgttcccacc acctgcttct ctagcaccct ccccgacact
     1141 gcctgacatg gatctggtgc ctggtggggg acagagccgg gctgagactt ggtccctgct
     1201 cagtgacagc accatggaca cgagatactc tgggaagaag tggccggtcc ccacggacac
     1261 aggaggggag gaagacaccg aggaccaggg cctgactgga gatgaagctg agccgttcct
     1321 ggaccagagt gctgctgtgg gcccaggggg accgaccaca cccaggaagc caccttccca
     1381 ccctcctccc cactatccag gggcaaaaag aaaaagaagc tctgccccac ctagaaagct
     1441 gctgtcagac aagccacagg acttccagat cagagtccag gtgatagagg ggcgtcagct
     1501 gcctggggtg aatattaagc ctgtggtcaa ggtcacagct gctgggcaga ccaagcgaac
     1561 tcggatccag aagggaaata gcccactctt caatgagact cttttcttca acgtatttga
     1621 ctctcccttg gagctgtttg atgagcccat cttcatcacg gtggtggact cacgctccct
     1681 taggacagat gccctccttg gcgagttccg gatggatgtg ggcaccgtgt acagagagcc
     1741 ccgacacgct tatctccgga agtggctgct gctctccgac cctgatgact tctctgcggg
     1801 ggccagaggc tacctcaaag ccagcctgtg tgtgctaggg cctggagatg aagctcccct
     1861 ggacaagaag gacccttctg aagacaaaga ggacattgaa ggcaacctgc ttaggcccac
     1921 cggtgtggcc cttcgaggag cccacttctg cctgaagctc ttccgggctg aggacttacc
     1981 acagatggac gatgctgtga tggacaacgt caagcagatc tttggctttg acagcaacaa
     2041 gaagaacttg gtggatccct ttgtggaagt cagctttgct gggaaaatgc tctgcagcaa
     2101 gatcctggag aagacagcta accctcagtg gaaccagaac atcaccttgc ctgcgatgtt
     2161 tccctctatg tgtgaaaaaa tgaggattcg tgtcatggac tgggaccgcc tcactcacaa
     2221 tgacactgtg gccaccacct acttgggtat gtcgaaaatc tctgctactg gaggagaaat
     2281 agaagtggac gacaacctgg gcttcctccc cacttttggg ccctgctatg tgaacctcta
     2341 tggcagtccc cgagagttca cgggcttccc agatccttat gcagagctca acacaggaaa
     2401 gggagaaggt gtggcttatc gaggcagggt tctgttgtcc ctggagacca aactggtgga
     2461 acacagtgag cagaaggtgg aagaccttcc tgctgatgat atcctgcgtg tggagaagta
     2521 cctgcggagg cgcaaatatt ccctctttgc tgccttctac tcagccacaa tgctgcagga
     2581 tgtggatgac gccatccagt tcgaggtcag cattgggaac tacgggaaca agttcgacac
     2641 cacctgcctg cccttggcct ccaccaccca gtacagccgg gcggtctttg atggatgcca
     2701 ctactattac ttgccttggg gcaacgtgaa gcccgtggtg gtgctgtcct catactggga
     2761 agacatcagc catcgaattg agatccaaaa ccagctcctc agggtcgctg accgcctgga
     2821 agctaacctg gagcaggtcc acttggctct gaaggcacag tgttcctccg aggatgtgga
     2881 cgccctggtg gctcagttga cagatgagct ccttgcagac tgcagccagc ccctgtgtga
     2941 catccacgag ataccttctg ccacccacct tgatcagtac ctgctccggc tacgcacccg
     3001 tcacctgagc cagatcaaag aagctgctct ggccttgaag cttggccata gtgagctctc
     3061 cactgccctg gagcaggccg aggactggct tctgcatctc cgtgccctgg ccgaggagcc
     3121 ccagaacagc ctgccggaca tcatcatctg gatgctgcag ggtgacaagc gtgtggccta
     3181 ccagcgggtg cctgcccatg aagtcctctt ctcccggcgg ggccccagct actgcggcag
     3241 gaactgtggg aagctgcaga ccatctttct gaaatatcct atggaaggta tgcctggggc
     3301 ccgaatgccc gtgcagattc ggatcaagct ctggttcggg ctctctgtgg atgagaagga
     3361 attcaatcag tttgctgagg gaaagctctc tgtctttgcc gaaacgtatg agaaccagac
     3421 aaagctggca ctggtcggga actggggcac aacgggcctc acctacccca agttttctga
     3481 tgtcacaggc aagatcaaac taccaaagga tagcttccgc ccctcagctg gctgggcctg
     3541 ggcaggagat tggttcgtgt gtccagagaa gaccctactc catgatgctg acgctggtca
     3601 tctgagcttc gtggaggagg tgttcgagaa ccagacccgg ctccctgggg gccagtggat
     3661 ttacatgagt gataactaca cagatgtgaa tggggagaag gtacttccga aggatgatat
     3721 tgaatgcccg ctgggctgga agtgggaaga tgaagaatgg tccacagacc tcaatcgggc
     3781 tgttgatgaa caaggctggg agtacagcat caccataccc ccagaccgaa agccaaaaca
     3841 ctgggtccct gttgagaaga tgtattacac tcacaggcgt cgacgctggg tccgactgcg
     3901 caggagggac ctcagccaga tggaagcact gaagaggcac aggcaggcag aggccgaggg
     3961 cgagggctgg gagtatgcct ctctcttcgg ctggaagttc cacctggagt accgcaagac
     4021 agatgccttc cgccgccgcc gctggcgccg ccgcatggag ccgctggaga agacgggacc
     4081 tgcagctgtg tttgccctcg agggggccct gggcggcatg gtggatgaca agagcgaaga
     4141 ctccatgtct gtttctaccc tgagctttgg cgttaacaga cccacaatct cctgcatctt
     4201 tgactatggg aaccgctacc atctgcgctg ctacctgtac caggcccgag acctgccagc
     4261 catggacaag gactccttct ctgatcccta cgccatcgtc tccttcctgc atcaaagcca
     4321 gaagacagtg gtggagaaga acaccctgaa tcccacctgg gatcaaaccc tcatcttcta
     4381 tgagattgag atcttcggcg agccagccag tatcgccgag catccgccct gcatcgtggt
     4441 ggagctctat gaccacgaca cctatggtgc tgacgagttc atgggtcgct gtatttgtca
     4501 gccaagcctg gaacggatgc cccgcctggc ctggttccca ctgacccggg gcagccagcc
     4561 tgccggcgag ctgctggcag cgttcgagct tatccagaga gagaagccag ccatccatca
     4621 catccctggc tttgagatgc atgaaacttc aaggattttg gatgagacgg aggacacaga
     4681 tttgccttac ccaccacctc aaagggaggc caacatctac atggttcccc agaacattaa
     4741 gccagctctc caacgtactg ccattgagat cctggcctgg ggcctgcgga acatgaagag
     4801 ctatcagatg gccagcatct cttcccccag cctcgtggtg gagtgtggtg ggcagacagt
     4861 gcagtcctgt gtcatcagaa accttcggaa gaaccccaac tttgatgtct gtactctctt
     4921 catggaagtg atgttaccca gagaggacct ctactgtcca cctattgtgg tcaaggtcat
     4981 tgataatcgc cagtttggcc gccggccagt tgtgggtcag tgtaccattc gctccctgga
     5041 gaacttcctg tgcgacccat actcagcaga gagtccatcc ccacagggtg gcccagatga
     5101 tgtgagctta ctcagccccg gggaagatgt gctgattgac atcgatgaca aggagcctct
     5161 cattcctgtc caggaggagg agttcattga ctggtggagc aaattctttg cttctgtcgg
     5221 ggagagggaa aagtgtggtt cctacctgga gaaggatttt gacaccttga aggtctatga
     5281 cacacagctg gagaatgtgg aggcttttgg aggcctgtcc gacttttgta acaccttcaa
     5341 gctctaccgg ggcaggactc aggaggagac agatgatcca tctgtcatcg gagaatttaa
     5401 gggtctcttc aaaatttatc ccctcccaga agatccagcc atccccatgc ccccgagaca
     5461 attccatcag ctggctgctc agggtcccca ggaatgcttg gtccgtattt atatcgtccg
     5521 agcatttggc ttacagccca aggaccccaa tggcaagtgt gacccttaca tcaagatctc
     5581 catagggaag aaatcagtga gtgaccagga taactacatc ccttgcaccc tggagcctgt
     5641 attcggaaag atgtttgaac tcacctgcac tctacctctg gagaaggacc tgaagatcac
     5701 actctacgat tatgacctgc tctccaagga tgagaagatt ggggagacgg tcatagacct
     5761 ggagaacagg ctgctgtcca agtttggggc tcgctgtggg ctcccacaga cctactgtgt
     5821 ctctggacca aatcagtgga gagatcagct ccgcccctcc cagctcctcc acctcttctg
     5881 ccagcagcac aggatcaagg cccccgtgta ccggacagac cgagtgacgt ttcaggataa
     5941 ggactacacc attgaggaga tagaggctgg cagactccca aacccacacc tgggcccagt
     6001 ggaggaacgc ttagccctgc atgtccttca gcaacaaggc ttggttcctg agcatgtgga
     6061 gtcacggcct ctttatagtc ctctgcagcc agatatcgag caggggaagc tacagatgtg
     6121 gattgacata tttccaaagg tgctgggccg gcctggacct cccttcaaca tcaccccacg
     6181 gaaagctaga aggttttttc tgcgttgtat tatttggaac acaaaagatg tgatcttgga
     6241 tgacctcagt ctcacggggg agaagatgag tgacatctat gtgaaaggct ggatggtggg
     6301 attcgaagaa cacaagcaga agacagatgt gcactaccgc tccctgggtg gggagggcaa
     6361 tttcaactgg aggttcgtgt ttcccttcga ctatctgcct gctgagcaag tctgtgctgt
     6421 tgccaagaag gatgccttct ggagactcga caagacagag agcaagatcc cagcacgtgt
     6481 ggtcttccag atctgggaca atgacaaatt ctcttttgat gactttctgg gctctctgca
     6541 gctggatctc aaccgaatgc ccaagccagc caagacggct gagaagtgct ccttggacca
     6601 gctggatgac accttccacc cagaatggtt tgtgtccctt tttgagcaga agacagtgaa
     6661 aggatggtgg ccttgtgtga cagaggaggg cgagaagaag atgttggcgg gcaagctgga
     6721 aatgaccttg gagattgttg cagaaagtga acatgaagag cggcctgctg gccaaggtcg
     6781 ggatgaaccc aacatgaatc cgaagctaga ggatccaagg cgccccgata cttctttcct
     6841 gtggttcacc tccccgtaca agaccatgaa gttcatcttg tggcgacgct tccggtgtgc
     6901 catcatcctc ttcatcatcc tcttcatcct cctgctcttc ctgggtgtct ttgtctacgc
     6961 cttcccgaac tacgctgcca tgaagctggt gaagcccttt cgatgagcat atgttaatta
     7021 acctaggtta taagcggccg cacccagctt tcttgtacaa agttggcatt ataagaaagc
     7081 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     7141 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     7201 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa ataatatcat
     7261 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     7321 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     7381 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     7441 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     7501 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     7561 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     7621 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     7681 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     7741 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     7801 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     7861 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     7921 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     7981 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     8041 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     8101 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     8161 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     8221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8881 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
