LOCUS       sequence-96317-e        9179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) and yeast codon-optimized yellow 
            fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1
            promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB787
FEATURES             Location/Qualifiers
     source          1..9179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2241..2261
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2243..2261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3184..3195
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3694..5343
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDVKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3760..3778)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6627..6814
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6682..6706)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7184..7203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7185..7203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7213..7230)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7213..7229)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7222..7244)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7374..7829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7461..7480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7671..7692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7960..8763)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8764..8984)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9010..9029)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9129..9151
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctct
     2281 gatgaacggt aagccaatga aggaaaagaa gtctaccaga agtgtcgaag acttcatggc
     2341 tgctatgcaa agagtctcta ctcaacacga aaaggaaacc taatcctgtt gaagtagcat
     2401 ttaatcataa tttttgtcac attttaatca acttgatttt tctggtttaa tttttctaat
     2461 tttaatttta atttttttat caatgggaac tgatacacta aaaagaatta ggagccaaca
     2521 agaataagcc gcttatttcc tactagagtt tgcttaaaat ttcatctcga attgtcattc
     2581 taatatttta tccacacaca caccttaaaa tttttagatt aaatggcatc aactcttagc
     2641 ttcacacaca cacacacacc gaagctggtt gttttatttg atttgatata attggtttct
     2701 ctggatggta ctttttcttt cttggttatt tcctatttta aaatatgaaa cgcacacaag
     2761 tcataattat tctaatagag cacaattcac aacacgcaca tttcaacttt aatatttttt
     2821 tagaaacact ttatttagtc taattcttaa tttttaatat atataatgca cacacactaa
     2881 tttattcatt aattttttat tgagtaggat ttgaaaatat ttggtatctt tgcaagatgt
     2941 ttgtatagag ggacaaagaa tcgtctttat tatggtcaag gctttacgtc ataatagttc
     3001 ctgcccagct cttctataat actttaaaga tctcttctcg tttgctccat ttggaagtct
     3061 cgcttacgtt tatgcgccca tacagacact caagatacac acttacatga acgtatacaa
     3121 atttactaac actacttgaa aatatgaacc acagtacatc atattaagac gtagtattcg
     3181 atgattgaag gccgcctccg cgaaatacct ttactgattt tgccggttaa tcgcatcgaa
     3241 atttcttcat cacaagaaag caaacaaatc gccaggccat tctacaagtt tccttttctt
     3301 atgaagatgt aaaagctact aaggcgtcat tactctagat gactcagttt agtctgacct
     3361 tctatagtat actaccctgg cgctatgatg atgagcggtt cttttattgc ggaaacgaaa
     3421 attccgggac cggcgaaatt tgcccggttt tgtccgtaac cggcttcatg agtcggcttc
     3481 aatagtagtt gaatacttat ttaaacagca gaacttaact cactcatcac gctgtttccg
     3541 ctgaattttc tcaaaatatc taagcagtca acaaatataa agaatattga aattgacagt
     3601 ttttgtcgct atcgattttt attatttgct gttttaaatc ttggtttaca ctccatccaa
     3661 actagaacta gtggatcccc cgggttaatt aacatggaag acgtcaaaaa cataaagaaa
     3721 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
     3781 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
     3841 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
     3901 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
     3961 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
     4021 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
     4081 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
     4141 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
     4201 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
     4261 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
     4321 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
     4381 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
     4441 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
     4501 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
     4561 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     4621 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
     4681 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
     4741 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     4801 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
     4861 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     4921 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     4981 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
     5041 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
     5101 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     5161 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     5221 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     5281 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc
     5341 gtgggtgggg gatcaatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg
     5401 gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt
     5461 gatgctactt acggtaaatt gaccttaaaa ttgatttgta ctactggtaa attgccagtt
     5521 ccatggccaa ccttagtcac tactttaggt tatggtttgc aatgttttgc tagataccca
     5581 gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa
     5641 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
     5701 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
     5761 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat cactgctgac
     5821 aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga agatggtggt
     5881 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
     5941 ccagacaacc attacttatc ctatcaatct gccttatcca aagatccaaa cgaaaagaga
     6001 gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg tattgatgaa
     6061 tctcgagaag catccaactt gaacatttcg agaaagctta ccatatcaac cccatcatgc
     6121 tctttcgaaa attcaaatag cacatccatt ccttcgcccg cttcctcatc tcaaagccac
     6181 actccaatga gaaacatgag ctcactctct gataacagcg ttttcagccg gaatatggaa
     6241 caatcatcac caatcactcc aagtatgtac caatttggtc agcagcagtc aaacagtata
     6301 tgtggtagca ccgttagtgt gaatagtctg gtgaatacaa ataacaaaca aaggatctac
     6361 gaacaaatca cgggtcctaa cagcaataac gcaaccaatg attatattga tttgctaaac
     6421 ctaaatgagt ctaacaagga aaaccaaaat cccgcaacgg cgcattacct caatgggggc
     6481 ccacccaaga caagcttcat taaccatgga atgttcccct cgccaactgg gaccataaat
     6541 agcggtaaat ctagcagtgc ctcatcttta atttcttttg gtatgggcaa tacccaagta
     6601 atatagggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
     6661 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     6721 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt
     6781 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa
     6841 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     6901 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     6961 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     7021 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     7081 cccctcacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     7141 tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata gtgagtcgta
     7201 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7261 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7321 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc
     7381 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7441 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7501 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7561 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7621 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7681 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7741 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7801 ttttaacaaa atattaacgt ttacaatttc ctgatgcggt attttctcct tacgcatctg
     7861 tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg
     7921 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     7981 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     8041 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     8101 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     8161 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     8221 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     8281 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     8341 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     8401 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     8461 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     8521 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     8581 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     8641 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     8701 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     8761 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     8821 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     8881 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
     8941 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagctg tggtatggtg
     9001 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     9061 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     9121 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
